Talisia esculenta (St. Hill, Radlk) : imunogenicidade em camundongos e efeito sobre parâmetros biológicos dos carrapatos Boophilus microplus (Canestrini, 1785) e Riphicephalus sanguineus (Latreille, 1806)

Abstract

The glucose-manose ligant lectina from Talisia esculenta seeds (TEL) vas investigated regarding to its immunogenicity after subcutaneous or intranasal applications, its potential role as immuneadjuvant against ovalbumin (OVA), the effect of specific sugars over these properties and lectin actions on biological parameters of ticks Boophilus microplus and Riphicephalus sanguineus, ectoparasites of bovine and dogs, respectively. The results obtained have shown tahat TEL is imunogenic when aplicated sucutaneosly, in a dose-dependent way. Imunogenicity pf the lectin was negatively affected by its previous incubation with the specific ligand sugar. TEL was able to increase immune response abti-OVA after subcutaneous application, but not after intranasal instilation. Treatment of female Boophilus microplus ticks with TEL have caused significant reduction on total leying eggs, reproductive and nutritional efficiency of the parasites, describing a polynomial type dose-response curve for the prior. These effects were significantly reduced by previous treatment of lectin with specific ligand sugars. Analysis by fluorescence microscopy revealed that TEL was able to link to cuticle of that tick. Significant effdets of the lectin against dos ticks brown R. sanguineus were not observed.