Uso de soro de leite bovino para elaboração de meios de cultura para Staphylococcus aureus

Abstract

We investigated the cultivation of Staphylococcus aureus ATCC 25923 in experimental media containing bovine whey. Liquid (whey broth – WB) and solid (whey agar – WA) media supplemented with sodium chloride (WB+NaCl and WA+NaCl) and yeast extract (WB+yeast and WA+yeast) were prepared. In WA media, the enrichment with yeast extract (0.1%) and water dilution (40%) (WA+H2 O+yeast) was tested. Commercial brain heart infusion broth (BHI) and Baird-Parker agar (BP) media were used as controls. The efficiency of experimental culture media was assessed by means of biomass determination and colony counts by using S. aureus ATCC 25923 strain. The bacterial growths in all experimental media were identical to those found in control media. In addition, grown bacteria colonies showed morphological characteristics compatible with ATCC S. aureus, especially in the WA+H2 O+yeast medium. Therefore, the bovine whey proved to be efficient as an alternative culture medium for S. aureus isolation and growth. Keywords. Staphylococcus aureus, bovine whey, experimental media.