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Type: Artigo de Periódico
Title: Studies on the secondary metabolites of a Pseudoalteromonas sp. isolated from sediments collected at the northeastern coast of Brazil
Authors: Arthaud, Isabelle D. B.
Rodrigues, Felipe A. R.
Jimenez, Paula C.
Montenegro, Raquel C.
Angelim, Alysson L.
Maciel, Vânia M. M.
Silveira, Edilberto R.
Freitas, Hozana P. S.
Sousa, Thiciana S.
Pessoa, Otília D. L.
Lotufo, Tito Monteiro da Cruz
Costa-Lotufo, Letícia V.
Keywords: Marine environment;Anticancer compounds;Gram-negative bacterium;Meio ambiente marinho;Componentes anticâncer;Bactérias Gram negativas
Issue Date: 2012
Publisher: Chemistry & Biodiversity,
Citation: ARTHAUD, Isabelle D. B.; RODRIGUES, Felipe A. R.; JIMENEZ, Paula C.; MONTENEGRO, Raquel C.; ANGELIM, A Alysson L.; MELO, Vania. M. M.; SILVEIRA, Edilberto R.; FREITAS, Hozana P. S.; SOUSA, Thiciana S.; PESSOA, Otília D. L..; LOTUFO, Tito Monteiro da Cruz; COSTA-LOTUFO, Letícia V. Studies on the secondary metabolites of a Pseudoalteromonas sp. isolated from sediments collected at the northeastern coast of Brazil. Chemistry & Biodiversity, Switzerland, v. 9, p. 418-427, 2012. Disponível em: Acesso em: 21 mar. 2023.
Abstract: Continuing search for anticancer compounds from the marine environment, we have studied microorganisms that inhabit intertidal sediments of the northeastern Brazilian coast. Of the 32 strains isolated, 13 were selected for biological evaluation of their crude extracts. The acetate extract obtained from a Gram-negative bacterium was strongly active against cancer cell lines with IC50 values that ranged from 0.04 (HL60 leukemia cells) to 0.26 μg/ml (MDA MB-435 melanoma cells). The bacterium was identified as a Pseudoalteromonas sp. based on 16S rRNA gene sequencing. A bioassay-guided fractionation of the active extract led to the isolation of prodigiosin, a well-known tripyrrole red pigment with immunosuppressive and anticancer activities. Further experiments with ErbB-2 overexpressing cell lines, including HB4a-C3.6 (moderate overexpression), HB4a-C5.2 (high overexpression), and the parental HB4a cell line, were performed. Prodigiosin was moderately active toward HB4a cells with an IC50 of 4.6 μg/ml, while it was 115 and 18 times more active toward HB4a-C3.6 cells (IC50 of 0.04 μg/ml) and HB4a-C5.2 (IC50 of 0.26 μg/ml) cells, respectively. These data suggest that, in spite of its previously described apoptosis-inducing properties, prodigiosin can selectively recognize cells overexpressing ErbB-2, which could be highly appealing in human breast cancer therapy.
ISSN: 1612-1880
Appears in Collections:LABOMAR - Artigos publicados em revistas científicas

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