Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil.

Abstract

Tenooutoofofiftyofreshoandorefrigeratedosamplesoofoshrimpo(Litopenaeus vannamei)ocollectedofromoretailersoino Natalo (Rioo Grandeo doo Norte,o Northeasterno Brazil)o testedo positiveo foro Vibrio parahaemolyticus.o Theo KanagawaotestoandomultiplexoPCRoassaysowereousedotoodetectoTDH and TRHohemolysinsoandotheotdh,otrhoando tlhogenes,orespectively.oAllostrainsowereoKanagawa4negativeoandotlh4positive.oAntibioticosusceptibilityotestingo waso doneo foro seveno antibioticso byo theo agaro diffusiono technique.o Fiveo strainso (50%)o presentedo multipleo antibiotico resistanceo too ampicillino (90%)o ando amikacino (60%),o whileo twoo strainso (20%)o displayedo intermediate4leveloresistanceotooamikacin.oAllostrainsowereosensitiveotoochloramphenicol.oIntermediate4levelo susceptibilityoand/ororesistanceotoootheroantibioticsorangedofromo10otoo90%,owithoemphasisoonotheoobservedo growingo intermediate4levelo resistanceo too ciprofloxacin.o Halfo ouro isolateso yieldedo ao multipleo antibiotico resistanceo indexo aboveo 0.2o (range:o 0.14–0.29),o indicatingo ao considerableo risko ofo propagationo ofo antibiotico resistanceothroughoutotheofoodochain.oo Key0words: multipleoantibioticoresistance.o