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http://repositorio.ufc.br/handle/riufc/67764
Tipo: | Artigo de Periódico |
Título: | Intraflagellar Transport (IFT) Complex in Leishmania spp.: in silico genome-wide screens and annotation of gene function. |
Autor(es): | Gouveia, João José de Simoni Vasconcelos, Elton José Rosas de Pacheco, Ana Carolina Landim Araújo Filho, Raimundo Maia, Allan Rodrigo Soares Kamimura, Michel Toth Costa, Marcilia Pinheiro da Viana, Daniel de Araújo Maggioni, Rodrigo Oliveira, Diana Magalhaes Costa, R. B. |
Palavras-chave: | Intraflagellar transport;Specie - Leishmania sp.;Flagellar motility;Trasporte intraflagelar;Espécie - Leishmania sp.;Mobililade Flagelar |
Data do documento: | 2007 |
Instituição/Editor/Publicador: | Genetics And Molecular Research |
Citação: | GOUVEIA, J.J.S.; VASCONCELOS, E.J.R.; PACHECO, A.C.L.; ARAÚJO-FILHO, R.; MAIA, A.R.S.; KAMIMURA, M.T.; COSTA, M.P.; VIANA, D.A.; COSTA, R.B.; MAGGIONI, R.. OLIVEIRA, D. M. Intraflagellar Transport (IFT) Complex in Leishmania spp.: in silico genome-wide screens and annotation of gene function. Genetics And Molecular Research, Ribeirão Preto, v. 6, n. 4, p. 766-798, 2007. |
Abstract: | Flagella are constructed and maintained through the highly conserved process of intraflagellar transport (IFT), which is a rapid movement of particles along the axonemal microtubules of cilia/flagella. Particles that are transported by IFT are composed of several protein subunits comprising two complexes (A and B), which are conserved among green algae, nematodes, and vertebrates. To determine whether or not homologues to members of the IFT complex proteins are conserved in Leishmania spp, we scanned genomes, transcriptomes and proteomes of Leishmania species in a search for putative IFT factors, which were then identified in silico, compared, cataloged, and characterized. Since a large proportion of newly identified genes in L. major remain unclassified, with many of these being potentially Leishmania- (or kinetoplastid-) specific, there is a need for detailed analyses of homologs/orthologs that could help us understand the functional assignment of these gene products. We used a combination of integrated bioinformatics tools in a pathogenomics approach to contribute to the annotation of Leishmania genomes, particularly regarding flagellar genes and their roles in pathogenesis. This resulted in the formal in silico identification of eight of these homologs in Leishmania (IFT subunits, 20, 27, 46, 52, 57, 88, 140, and 172), along with others (IFTs 71, 74/72, and 81), as well as sequence comparisons and structural predictions. IFT, an important flagellar pathway in Leishmania, begins to be revealed through screening of trypanosomatid genomes; this information could also be used to better understand fundamental processes in Leishmania, such as motility and pathogenesis. |
URI: | http://www.repositorio.ufc.br/handle/riufc/67764 |
ISSN: | 1676-5680 |
Aparece nas coleções: | LABOMAR - Artigos publicados em revistas científicas |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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2007_art_jjmgouveia.pdf | 1,93 MB | Adobe PDF | Visualizar/Abrir |
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