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http://repositorio.ufc.br/handle/riufc/66484
Type: | Artigo de Periódico |
Title: | Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury |
Authors: | Freitas, Flavia Priscila Santos Porto, Marcella Leite Tranhago, Camilla da Penha Piontkowski, Rogerio Miguel, Emilio de Castro Miguel, Thaiz Batista Azevedo Rangel Martins, Jorge Luiz Nascimento, Kyria Santiago do Balarini, Camille de Moura Cavada, Benildo Sousa Meyrelles, Silvana dos Santos Vasquez, Elisardo Corral Gava, Agata Lages |
Keywords: | Acute kidney injury;Ischemia reperfusion;Renal function;Plant lectin;Dioclea violacea |
Issue Date: | 2015 |
Publisher: | American Journal of Translational Research |
Citation: | FREITAS, Flavia P. S. et al. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. American Journal of Translational Research, [s. l.], v. 7, n. 12, p. 2573–2588, 2015. |
Abstract: | Acute kidney injury (AKI) is characterized by rapid and potentially reversible decline in renal function; however, the current management for AKI is nonspecific and associated with limited supportive care. Considering the need for more novel therapeutic approaches, we believe that lectins from Dioclea violacea (Dvl), based on their antiinflammatory properties, could be beneficial for the treatment of AKI induced by renal ischemia/reperfusion (IR). Dvl (1 mg/kg, i.v.) or vehicle (100 µL) was administered to Wistar rats prior to the induction of bilateral renal ischemia (45 min). Following 24 hours of reperfusion, inulin and para-aminohippurate (PAH) clearances were performed to determine glomerular filtration rate (GFR), renal plasma flow (RPF), renal blood flow (RBF) and renal vascular resistance (RVR). Renal inflammation was assessed using yeloperoxidase (MPO) activity. Kidney sections were stained with hematoxylin-eosin to evaluate morphological changes. Intracellular superoxide anions, hydrogen peroxide, peroxynitrite, nitric oxide and apoptosis were analyzed using flow cytometry. IR resulted in diminished GFR, RPF, RBF, and increased RVR; however, these changes were ameliorated in rats receiving Dvl. AKI-induced histomorphological changes, such as tubular dilation, tubular necrosis and proteinaceous casts, were attenuated by Dvl administration. Treatment with Dvl resulted in diminished renal MPO activity, oxidative stress and apoptosis in rats submitted to IR. Our data reveal that Dvl has a protective effect in the kidney, improving renal function after IR injury, probably by reducing neutrophil recruitment and oxidative stress. These results indicate that Dvl can be considered a new therapeutic approach for AKI-induced kidney injury. |
URI: | http://www.repositorio.ufc.br/handle/riufc/66484 |
ISSN: | 1943-8141 |
Appears in Collections: | DEMM - Artigos publicados em revista científica |
Files in This Item:
File | Description | Size | Format | |
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2015_art_fpsfreitas.pdf | 2,24 MB | Adobe PDF | View/Open |
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