Integridade da mucosa duodenal de camundongos: efeitos de estímulos (acidez, pepsina e sais biliares) e de agentes protetores tópicos

Abstract

The gastrointestinal tract (GT), in its relevant aspects, has as its main characteristic the action in the process of nutrient absorption, however, in addition, it has the function of important agents in the barrier against the systemic entry of nutrients. With regard to pathological conditions, intestinal barrier dysfunction is associated with Functional Dyspepsia (FD), affecting from 10% to 30% of general population and being present in around 40% of the Brazilian population. With respect to its point of origin, data increasingly emerges that the duodenum is a key region underlying its pathophysiology. The purpose of the project is to assess possible effect of acid, pepsin and bile salts on the duodenal mucosa present in mice, as well as investigating the protective effect of treatments. For this, duodenal biopsies from male Swiss mice (20 to 30g and with a significant N of 6 per group) were used. In order to evaluate the resistance electrical transepithelial duodenal mucosa was measured the acids (pH7.4; pH 2; pH 1,75; pH 1,5; pH 1,25; pH 1) in the duodenal epithelium. Was measured also if the damage that was found at pH 1,5 was becoming permanent . The made the analyze too the effect of acid (pH 1.5) with the addition to pepsin, biliary salt TDAC (taurodeoxycholic acid) and GUDAC (Glucoursodeoxycholic Acid). Finally, it evaluated the topical protective effect of the compounds Alginate, Angico Gum (AG), Cashew tree Gum (CTG) and Sucralfate in these changes. Thus, the acid-dependent effect on duodenal RETE was observed, increasing as it intensifies in the fall of the solution of the solutions used. Prolonged exposure to acid (pH 1,5) does not make the harm permanent that's because restores it when tissue pH has been observed to its physiological pH. Both isolated and increased bile acid, as well as pepsin and TDAC with to acid (pH1,5), do not cause damage to bile acid, as well the bile salt, did not prevent duodenal RETE from falling. And finally, the acid in the duodenal mucosa does not prevent the RETE drop with of the recommended bioprotectors occasionally. Therefore, studies are needed to clarify the acid pathway in damage related to the duodenal epithelium and thus search for new treatments aimed at duodenal disorders.