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http://repositorio.ufc.br/handle/riufc/62906
Tipo: | Artigo de Periódico |
Título : | A novel bacterial carboxylesterase identified in a metagenome derived‑clone from Brazilian mangrove sediments |
Autor : | Araujo, Francisco J. Hissa, Denise Cavalcante Silva, Gabrielly O. Antunes, André Saraiva Leão Marcelo Nogueira, Vanessa L. R. Gonçalves, Luciana R. B. Melo, Vânia M. M. |
Palabras clave : | Metagenomic library;1.4-Butanediol diacrylate esterase;Family VIII;Porticoccus;Heterologous expression |
Fecha de publicación : | 2020 |
Editorial : | Molecular Biology Reports |
Citación : | ARAUJO, Francisco J. et al. A novel bacterial carboxylesterase identified in a metagenome derived‑clone from Brazilian mangrove sediments. Molecular Biology Reports, [s. l.], v. 47, n. 5, p. 3919-3928, maio 2020. |
Abstract: | A functional screening of 1152 clones from a plasmid library constructed with DNA extracted from Brazilian mangrove sediments revealed 3 positive clones for ester-hydrolyzing enzymes, or about one lipolytic gene per 1.2 Mb DNA, which corroborates the idea that oil-contaminated mangroves are a good source of novel microbial lipases/esterases. The partial sequence of the clone LipG7 (1179 bp) showed 30.2% of predicted structure identity with a known esterase, confrming LipG7 as a new member of family VIII esterases. LigG7 shared 80% sequence identity with 1,4-butanediol diacrylate esterase from the Gammaprotebacteria Porticoccus hydrocarbonoclasticus, suggesting it belongs to the Porticoccaceae family. LipG7 was heterologously expressed in Escherichia coli Rosetta-Gami DE3; the purifed recombinant enzyme exhibited a predicted molecular weight of 45.2 kDa and exceptional activity towards 4-nitrophenyl butyrate, compared with other recombinant esterases, highlighting its enormous potential for biological applications. |
URI : | http://www.repositorio.ufc.br/handle/riufc/62906 |
Derechos de acceso: | Acesso Aberto |
Aparece en las colecciones: | DBIO - Artigos publicados em revista científica |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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2020_art_fjaraujo.pdf | 1,32 MB | Adobe PDF | Visualizar/Abrir |
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