O uso de linhagens leucêmicas e a sua importância na oncologia experimental

Abstract

Experimental oncology is based on a model of study through cell culture, a technique frequently used for the study of cancer biology and for tests of new anticancer therapies. This model provides an appropriate system for researching the effects of drugs and toxic compounds on cells, mutagenesis and carcinogenesis, in drug screening and development. In addition the cell lines allows consistent and reproducible results, becoming one of the main tools used in experimental oncology, with the advantage of being derived from patients and easily manipulated in the laboratory. The use of cell lines is fundamental in the research related to the study of new antineoplastic drugs for the treatment of patients with chronic myeloid leukemia (CML) who are still refractory to the currently available therapies. Thereby, the understanding of the mechanisms of action and resistance and sensitivity patterns of these chemotherapies through the use of these lineages can make cancer therapy more efficient and specific. Thus, we highlight in this chapter the applications of cell lines as a study model for the various biological aspects of cancer in Experimental Oncology.