Purificação e caracterização físico-química de lectina isolada de sementes de Dalbergia ecastophyllum (l.) Taub.

Abstract

The seeds of Dalbergia ecastophyllum (L.) Taub, a species belonging to the Fabaceae family, Papilionoideae subfamily, Dalbergieae tribe, and genus Dalbergia, have a specific N-acetyl-D-glucosain/melibiosis/L-rhamnose lectin, which agglutinates erythrocytes native or trypsin-treated rabbit. The lectin, named DeL, was purified in one step by Chitin matrix affinity chromatography, the first lectin of its genus to be purified. The entire purification process was monitored by SDS-PAGE, specific haemagglutination activity and molecular exclusion chromatography. Where the purified DeL presented an electrophoretic profile composed of a band of approximately 20 kDa of apparent molecular mass. In addition, DeL does not demonstrate stability over a wide range of pH or temperature. It also has no toxicity against Artemia sp. Nauplii, nor is it glycoprotein too.