Avaliar a reprodutibilidade de método cromatográfico para o isolamento de ácido anacárdico presente no líquido da castanha de caju (LCC)

Abstract

The cashew is a fruit belonging to the family Anacardiácea, consists of the peduncle part of the pulp of the fruit (pseudofruit) and the chestnut which is the true fruit. Cashew nut shell is a raw material for obtaining chestnut husk liquid (CNSL), a dark brown viscous substance with important applications in the chemical and pharmaceutical industry. CNSL is rich in long chain and unsaturated phenolic compounds, such as cardol, cardanol, methyl-cardol and anacardic acids. Anacardic acids are phenolic compounds, presenting lipid character resulting from their aliphatic side chain. It is considered a phytochemical of interest due to its wide biological activity, which include microbicidal activities, insecticides and molluscicides. Recent studies show positive results of the use of anacardic acids (AnAc) in treatments and prevention of diseases. Therefore, studies are needed to develop methods capable of isolating these compounds in the CNSL. The present work aims to evaluate the reproducibility of chromatographic method to isolate, identify and evaluate the productivity of AnAc present in the CNSL. Samples of natural CNSL were solubilized in methanol and injected into a High Performance Liquid Chromatography (HPLC-UV / VIS) monitored at a wavelength of 280 nm, equipped with a preparative scale C18 column using as the mobile phase methanol, H2O and acetic acid in isocratic mode. The productivity was 0.6791, 0.4416 and 1.325 g.h-1 by each g of adsorbent for the anacardic acids (15:1), (15:2) and (15:3), respectively. The chromatographic method used was adequate for the isolation of the anacardic acids present in the CNSL. The isolation of the analytical standards was obtained with high productivity and low solvent consumption.