Endometriose peritoneal experimental em ratas: avaliação da dor, fertilidade, estresse oxidativo, inflamação e sua resposta aos nutracêuticos ômega-6/3 e ômega-9/6

Abstract

Endometriosis is defined as the presence of endometrial tissue (glands and stroma) outside the uterine cavity. Objective: Evaluate the effect of the nutraceuticals omega-6/3 and omega-9/6 on pain, fertility, inflammation and oxidative stress associated with endometriosis. Methods: In this controlled experimental study, peritoneal endometriosis was induced surgically in albino Wistar rats by implanting two fragments from the right hemiuterus, one near the right ovary and one in the anterior abdominal wall. The distribution of wet and dry weight was evaluated at four moments (days 1, 7, 14 and 21). Groups of 8 animals were submitted to treatment with either β-stradiol 10 mg/kg/day, medroxyprogesterone acetate 5 mg/kg every 3 days, omega-6/3 1.2 g/kg/day, omega-9/6 1.2 g/kg/day, or meloxicam 0.8 mg/kg/day, and compared with two control groups (sham surgery, and endometriosis + 0.9% saline). Pain was quantified with the abdominal contortion test for the two control groups, the omega-6/3 group, the omega-9/6 group, the medroxyprogesterone acetate group and the meloxicam group, at the described dosages. Fertility was expressed as the number of developing embryos in the left hemiuterus for the two control groups, the omega-6/3 group, the omega-9/6 group and the meloxicam group, at the described dosages. Inflammation was proxied by the plasma level of Creactive protein for the two control groups, the omega-6/3 group and the omega-9/6 group, at the described dosages. Oxidative stress was represented by plasma and tissue glutathione and TBARS levels for the two control groups, the omega-6/3 group and the omega-9/6 group, at the described dosages. Results: The greatest mean wet and dry implant weight (± SEM) was observed on day 14. This group was used as reference when comparing the treatments. In the pain group there was a significant reduction of the abdominal contortions, in the inflammation there was a significant reduction of the C reactive protein and in the oxidative stress group there was a reduction of TBARS and a significant increase of the glutathione. Conclusion: Animals ingesting omega-6/3 and omega-9/6 displayed lower implant weight and less pain, inflammation and oxidative stress, but fertility was not improved. Key words: Endometriosis. Dietary Supplements. Fertility. Pain. Oxidative stress. Fatty Acids, Omega-3. Inflammation.