Efeito antimicrobiano de dentifrício de própolis vermelha brasileira sobre STREPTOCOCCUS MUTANS: estudo in vitro.

Abstract

Purpose: To compare the antibacterial effects of a Brazilian red propolis dentifrice, the extract and different commercial dentifrices against S.mutans in vitro. Materials and Methods: The Brazilian red propolis extract, the Brazilian red propolis dentifrice and the commercial dentifrice analyzed. Strains of S. mutans were used in the present study. For the evaluation of the antimicrobial activity the microdilution method was used in culture broth, according to Standard M07-A10 of the Clinical and Laboratory Standard Institute (CLSI, 2015). The strain was activated by incubation at 37 ° C overnight in Brain Heart Infusion (BHI) culture medium, and in anaerobic jar, using the candle method. To the wells of the microplates were added 100μL of BHI broth, 20μL of the substances tested (experimental groups), at concentrations ranging from 100 μL / mL to 0.0488 μL / mL, and 80 μL / mL of the standardized microbial suspension (106 CFU / mL). The microplates were incubated for 24 hours in a bacteriological oven at 37 ° C. Propolis extract and dentifrice showed significant antimicrobial activity against S.mutans up to the concentration of 0,3906 μg/mL. The common dentifrice showed significant antimicrobial activity against S.mutans up to the concentration of 0.0976 μg / mL. It was concluded that all the groups observed had antimicrobial activity against S.mutans. There was no difference between the propolis extract, the dentifrice incorporated with the extract and the common dentifrice.