Construção de molécula de RNA de interferência para silenciar genes que promovem a formação de biofilmes por bactérias do gênero Staphylococcus

Abstract

Staphylococcus aureus and Staphylococcus epidermidis are causative agents of common infections in humans and are important pathogens responsible for nosocomial infections associated with the use of implantable devices. One of its main factors of virulence and biofilm. Biofilm formation is regulated by a complex genetic machine, with sarA, operon ica and agr genes being the most studied. The indiscriminate use of antibiotics over time has generated an explosion of "superbugs", especially bacteria of the genus Staphylococcus, multiresistant to various antibiotics. Currently, many studies have documented the strategy to combat mechanisms of resistance in microorganisms. Interference RNA is a biological process that causes silencing of specific mRNA molecules and has been used as a tool for both the study of gene functions and gene silencing. Despite the many barriers that prevent the application of the technique to clinical use, researchers have overcome some problems from the use of hybrid RNADNA molecules, called siHybrids. This work aimed to construct hybrid RNA-DNA molecules (siHybrids) to silence an action of the icaA gene and the agr gene, thus preventing a biofilm formation. The results showed that biofilm production systems as the new therapeutic strategy for the inhibition of biofilm formation. This study is a program of efficacy of RNA: DNA hybrids against Staphylococcus spp.