Please use this identifier to cite or link to this item: http://repositorio.ufc.br/handle/riufc/23099
Type: Artigo de Periódico
Title: Clinical evaluation, biochemistry and genetic polymorphism analysis for the diagnosis of lactose intolerance in a population from northeastern Brazil
Authors: Ponte, Paulo Roberto Lins
Medeiros, Pedro Henrique Quintela Soares de
Havt, Alexandre
Caetano, Joselany Áfio
Cid, David A.C. CidI
Prata, Mara de Moura Gondim
Soares, Alberto Melo
Guerrant, Richard L.
Mychaleckyj, Josyf
Lima, Aldo Ângelo
Keywords: Intolerância à Lactose;Gerenciamento Clínico;Lactose Intolerance
Issue Date: Feb-2016
Publisher: Clinical Science
Citation: PONTE, P. R. L. et al. Clinical evaluation, biochemistry and genetic polymorphism analysis for the diagnosis of lactose intolerance in a population from northeastern Brazil. Clinical Science, v. 71, n. 2, p. 82-89, fev. 2016.
Abstract: OBJECTIVE: This work aimed to evaluate and correlate symptoms, biochemical blood test results and single nucleotide polymorphisms for lactose intolerance diagnosis. METHOD: A cross-sectional study was conducted in Fortaleza, Ceará, Brazil, with a total of 119 patients, 54 of whom were lactose intolerant. Clinical evaluation and biochemical blood tests were conducted after lactose ingestion and blood samples were collected for genotyping evaluation. In particular, the single nucleotide polymorphisms C>T-13910 and G>A-22018 were analyzed by restriction fragment length polymorphism/polymerase chain reaction and validated by DNA sequencing. RESULTS: Lactose-intolerant patients presented with more symptoms of flatulence (81.4%), bloating (68.5%), borborygmus (59.3%) and diarrhea (46.3%) compared with non-lactose-intolerant patients (p<0.05). We observed a significant association between the presence of the alleles T-13910 and A-22018 and the lactose-tolerant phenotype (p<0.05). After evaluation of the biochemical blood test results for lactose, we found that the most effective cutoff for glucose levels obtained for lactose malabsorbers was <15 mg/dL, presenting an area under the receiver operating characteristic curve greater than 80.3%, with satisfactory values for sensitivity and specificity. CONCLUSIONS: These data corroborate the association of these single nucleotide polymorphisms (C>T-13910 and G>A-22018) with lactose tolerance in this population and suggest clinical management for patients with lactose intolerance that considers single nucleotide polymorphism detection and a change in the biochemical blood test cutoff from <25 mg/dL to <15 mg/dL.
URI: http://www.repositorio.ufc.br/handle/riufc/23099
ISSN: 1807-5932 Print
1980-5322 On-line
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