Avaliação in vitro da interferência de lectinas vegetais e do diterpeno casbano isolado de Croton nepataefolius sobre o crescimento de formas planctônicas e biofilmes de Pseudomonas aeruginosa

Abstract

This work shows the biological activities of lectins isolated from Vatairea macrocarpa and Vatairea guianensis seeds and the vegetable compound diterpen casban, isolated from Croton nepetaefolius on the growth of Pseudomonas aeruginosa (ATCC 9027) that causes otites externa. The in vitro activity of the two lectins and vegetable compound casbane diterpene were compared on cultures of P. aeruginosa in polystyrene microplates. The bacterial cells were tested such in planktonic as in biofilm forms. The lectins tested were not capable to inhibit the growth and biofilm production of P. aeruginosa in the experimental conditions. On the other hand, the casbane diterpene was capable to inhibit the growth of planctonic forms of P. aeruginosa at the concentrations of 500, 250 and 125 µg/mL. However, the inhibition of biofilm production was not observed at the same concentrations. The casbane diterpene isolated from Croton nepetaefolius can be used, after the realization of other studies, as an antibiotic biotechnological tool on planktonic forms of P. aeruginosa