Ação anti-inflamatória e antioxidante do mix de óleos ômega 9, 6 e 3 de baixa relação ômega-6/ômega-3 e elevada relação ômega-9/ômega-6 após exodontia em ratos

Abstract

Oil fatty acids omega-3 and omega-9 have anti-inflammatory and antioxidant activities, while the omega-6 has pro-inflammatory action. The aim of this study was to evaluate the presence of antioxidant and anti-inflammatory effects with high nutraceutical ratio of omega-9: omega-6, antioxidant properties, and low ratio omega-6: omega-3, anti-inflammatory properties three days after tooth extraction in rats. Thirty-two young Wistar rats with 270-310g were randomly divided into four groups of 8 animals: Group Sham, Saline Group, Isolipid Group, and Mix Test Group. The animals received 0.9% NaCl, Sham Group and Salina Group, or Isolipid Mix (alpha-linolenic acid - ALA) mixture containing omega-6: omega-3 oils (ratio 8:01) and omega-9: omega-6 (ratio 0.4:1), Isolipid group, or Mix Test (alpha-linolenic acid - ALA, docosahexaenoic acid - DHA, eicosapentaenoic acid - EPA) omega-6: omega-3 (ratio 1.4: 1) and omega-9: omega -6 (3.4:1 ratio), Mix Text Group by gavage 1.2g solutions for kg/day for four days before and three days after the extraction of the left mandibular first molar. Evaluation of edema generated by the surgical procedure and collected the perialveolar the site of tooth extraction and dental alveolar mucosa itself for laboratory testing were performed: myeloperoxidase, osteoclast count, concentrations of TNF-α and IL-1β, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS). The Mix Test induced a significant decrease on post-operative edema, myeloperoxidase , osteoclasts count , GSH in blood and tissue, on tissue and plasma TBARS and on the TNF-αconcentration, but did not show significant difference on the IL-1β concentration at the third post-operative day. The gavage with the Mix Test showed anti-inflammatory and antioxidant actions after molar tooth extraction in rats.