Efeito do suco de acerola (Malpighia emarginata DC) no metabolismo oxidativo de camundongos Swiss submetidos a exercício agudo de natação.

Abstract

Malpighia emarginata DC. known as “Acerola”, is a widely consumed fruit in Brazil for its high antioxidant content. The objective of this study was to evaluate the activity of antioxidant enzymes (SOD and GPx) in erythrocytes and lipid peroxidation in livers of mice fed with acerola juice (BRS 238-frutacor) and subjected to exhaustive swimming session. The levels of antioxidants were determined in the juice and in the pulp. Water, acerola juice or ascorbic acid were administered (intragastrically) to mice for 30 consecutive days. The animals were divided into 4 groups (n = 10): water, water + exercise, exercise and juice ascorbic acid + exercising. After 30 days, the mice were subjected to an exhaustive session of swimming and an hour later an aliquot of blood was withdrawn for analysis of the enzymes SOD and GPx in erythrocytes. Then, the mice were euthanized and had their livers removed for analysis of lipid peroxidation. The values of antioxidant: anthocyanins, flavonols, vitamin C, polyphenols and total antioxidant activity for juice and pulp were respectively: 18.9 ± 0.004 mg / 100 g, 34.6 ± 0.002 mg / 100 g, 11.3 ± 0.003 mg / 100 g and 22.3 ± 0.00 mg / 100 g, 718 ± 0.033 mg / 100 g and 1453 ± 0.088 mg/100 g, 659 ± 4 mg/100 g and 1153.34 ± 0 mg/100 g, and 18, 5 ± 1.5 mM Trolox / ml and 36.24 ± 0.65 mM Trolox / mL. The activity of the enzymes SOD and GPx groups in water, exe + juice + and AA + exe exe ranged in U / g Hb, respectively, 875.7, 873.7, 920, 447.4 and 52.5, 55, 78, 54.34 and 68.32. The results show that such activities were not changed showing that exercise did not cause oxidative damage and that the acerola juice did not influence the activity of these enzymes. The basal oxidative stress was attenuated by ascorbic acid when it was determined the activity of SOD. The hepatic lipid peroxidation in groups water, exe + juice + and AA + exe in nmol / g tissue were respectively 179.5, 227.2, 158.8 and 188.7, revealing stress in response to exercise and treatment with acerola juice induced greater protection than the control group. It is concluded that oxidative damage generated by the exercise seems to depend on the target tissue, enzymes evaluated and the animal under study.