Ação enteroprotetora do ácido centipédico, isolado de Egletes viscosa less, em modelo de lesões intestinais induzidas por Indometacina em ratos

Abstract

Egletes viscosa less, popularly known as ―macela‖, is a vegetable species used in folk medicine, especially in the treatment of gastrointestinal diseases. The centipedic acid, a diterpene that is present in essential oil was analyzed this work, to verify their involvement in the of intestinal lesions caused by indomethacin. From the model of intestinal ulcers induced by respective NSAIDs, in concentration of 10 mg/kg, oral tract, administered for three days, there were no disorder kidney (urea and creatinine) neither disorder of liver (AST and ALT). The treatment with AC (50 mg/kg) decreased significantly the number of longitudinal ulcers (>5mm), but not the specific number of ulcers (<5mm). AC showed antioxidant activity by decreasing of MDA and MPO levels and restoration of NP-SH and catalase levels. In intestinal cultured cells (IEC-6), AC (12,5, 25, 50 and 100 μM ) showed activity pro-migratory, and their combination (25, 50 and 100 μM) with indomethacin 250 μM, but not with 1000 μM, change toxicity of indomethacin. AC (6,25, 12,5, 25, 50, 100 and 200 μM) alone didn´t show statistical difference on cell proliferation of IEC-6. However, in combination. the diterpene (12,5, 25, 50, 100 μM) significantly protected IEC- 6 of toxicity by indomethacin (250 and 1000 μM). The present results show, therefore, that AC influenced migration of IEC-6 cells. Moreover, the substance studied showed skill in cell proliferation. The data suggest that the acid diterpene centipédico has the potential enteroprotetor possibly related to a particular antioxidant mechanism and that could be an effective therapeutic agent to treat of ulcers and intestinal side effects caused by NSAID.