Desenvolvimento de matriz desmineralizada de osso cortical suíno: influência da peroxidação sobre morfologia e grau de intumescimento

Abstract

The porcine source attracts the development of xenogeneic natural biomaterials, due to the availability of collagen in soft tissues, but the use of the demineralized organic matrix of cortical bone remains poorly explored. The aim of this pilot study was to develop a demineralized porcine cortical bone matrix and to analyze the influence of the degree of peroxidation on the morphology and degree of swelling. Fresh pork ribs were processed in eight protocols: pretreatment for 3h in hydrogen peroxide 3% (G1), 10% (G2), 20% (G3) or 30% (G4) and demineralization for 14 days, or post-treatment treatment in 3% (G5), 10% (G6), 20% (G7) or 30% (G8) hydrogen peroxide, using native bone as a control. All samples were cleaved, dried and sterilized, followed by processing and staining for histological and histomorphometric analysis in Image J software or for testing the degree of swelling in distilled water. There were changes in typical lamellar bone morphology in all tested groups; pretreatment had an increase in porosity (<250μm) as the concentration increased and maintained a matrix closer to the original, while posttreatment had a more evident increase in porosity (>250μm) and fusion of bone channels mimicking a mesh, as the concentration increased. Permeable spaces in the bone matrix were greater in histomorphometry and hydrophilicity was greater and more persistent in the swelling test at post-treatment compared to pre-treatment. The 3% and 10% hydrogen peroxide concentrations were less aggressive than the 20% and 30% ones, being more indicated in pre-treatment to generate a membrane.