Efeitos do bioativo troxerutina no modelo de periodontite induzida por ligadura em camundongo Swiss

Abstract

Periodontitis is a chronic immune-inflammatory disease that affects the supporting and protective tissues of the teeth. The biological activity of flavonoids has emerged as a promising alternative against inflammatory processes. Troxerutin, a semi-synthetic flavonoid derived from rutin and widely found in various foods, has emerged as a promising bioactive since studies have revealed its antioxidant, anti-edematogenic, and anti-inflammatory effects. For this purpose, 40 Swiss mice were randomly allocated into five groups. Periodontitis was induced by placing a 3.0 cotton thread in the first lower molars of Swiss mice anesthetized with Ketamine (100mg/kg; i.p.) and Xylazine (10mg/kg; i.p.). The control groups included animals that had not undergone ligature-induced (Naive) and animals that had undergone periodontitis induction and received oral administration of saline solution (Salina) once a day until euthanasia. On the 11th day, all the animals were euthanized by exsanguination under anesthesia. The hemiarches were removed and processed for morphometric and histopathological analyses (HE) and RANKL and OPG gene expression investigation by realtime PCR (RT-PCR). The gingival tissue around the first molars was collected to determine the lipid peroxidation marker, malondialdehyde (MDA), and the antioxidant marker, reduced glutathione (GSH) concentrations. Serum bone alkaline phosphatase levels were assessed using blood collected from the orbital plexus on the 11th day. The results showed that Troxerutin effectively preserved alveolar bone at 150mg/kg. In addition, this dose reduced the activity of bone alkaline phosphatase induced by periodontitis, which is associated with higher concentrations of reduced glutathione (GSH) and indicates a favorable redox environment. In addition, Troxerutin (150 mg/kg) reduced lipid peroxidation, as evidenced by a decrease in malondialdehyde (MDA) concentration. These findings suggest that Troxerutin plays an osteoprotective role in induced periodontitis, possibly mediated by its anti-inflammatory and antioxidant properties, contributing to maintaining bone homeostasis in inflammatory conditions.