Presença de Pseudomonas e Enterococcus em galerias pluviais da cidade de Fortaleza, Ceará

Abstract

The water present in the urban centers, such as rivers, lakes and seas are harmed due to intense and unrestrained use of this resource, causing various effects with large impacts. This research aimed to the microbiological analysis of two galleries and points of sea water near the outlet of rainwater canals in the city of Fortaleza-CE. Five consecutive samples, one per week, were collected in the months of May and June 2013, always in the morning. The temperature of the samples was measured in the same place and the samples were processed immediately in the microbiology laboratory (LAMAP), where salinity and pH were measured. To calculate the MPN of Pseudomonas/100mL was followed the CETESB standard for recreational waters. For isolation of Enterococcus, the membrane filter technique was used and the Enterococcus count was expressed as CFU/mL. The susceptibility profiles of the strains of P. aeruginosa was carried out with the following antimicrobial: 30μg amikacin (AMI), 10mg imipenem (IPM), 10mg Gentamicin (GEN), 30μg Tobramycin (TOB), 30μg aztreonam (ATM) and 30μg ceftazidime (CAZ). Thirty-seven strains were identified as Pseudomonas aeruginosa, forty-four as Pseudomonas ssp. and ninety as Enterococcus. The MPN of Pseudomonas/100mL ranged from < 1.8 x 102 to 1.6 x 104 and CPP Enterococcus ranged from 1.0 x 102 CFU/mL to 9.0 x 103 CFU/mL. From the identified strains, Enterococcus was the most numerous in the total isolated of the galleries and sea points samples. The isolated strains of P. aeruginosa do not present any resistance of any antibiotic tested. It follows that: Pseudomonas and Enterococcus were identified in the Maceió river gallery, Meireles beach gallery and sea points near the galleries outlets of the rainwater; in galleries there was more isolation of strains of Enterococcus than in samples of sea water; Among the studied points, the largest number of identified isolated strains were from bacteria of genus Enterococcus; Strains of P. aeruginosa were susceptible to all tested antimicrobials.