Chromobacteriaceae: uma análise filogenética multilocus

Abstract

The family Chromobacteriaceae (Betaproteobacteria) is a group containing species of great biotechnological importance, including a Chromobacterium violaceum species. Required to advance bioinformatics techniques, new species are constantly included in this family. Since the family was proposed, despite its importance and steady growth, no new study has focused on its phylogeny. In this work, bioinformatics tools (BLAST, MAFFT and RAxML-NG) were used to build two phylogenetic trees, one based on amino acid sequences of 19 housekeeping genes (Adk, AtpG, EF-G, FusA, GltX, Gmk , GyrA, GyrB, L5, LipA, MurC, MurE, PgK, RpoB, S2, S3, SecY, ThrRS, UvrD), the other based on nucleotide sequences of 16S/23S rRNA genes. According to the trees, Chromobacteriaceae family is divided into two large branches, one containing the genera Paludibacterium, Gulbenkiania, Pseudogulbenkiania, Vogesella, Aquitalea and Chromobacterium (Clado 1), the other containing the genera Aquaspirillum, Microvirgula, Laribacter, Leeia, Iodobacter, Formivibrio, Andreprevotia, Chitiniphilus, Jeongeupia, Chitiniliyticum, Deefgea and Chitinibacter (Clado 2). It was also revealed a possible taxonomic inconsistency of the strain Pseudogulbenkiania ferrooxidans EGD-HP2 in both trees.