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http://repositorio.ufc.br/handle/riufc/54436| Tipo: | Artigo de Evento |
| Título: | Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens |
| Título em inglês: | Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens |
| Autor(es): | Rios, Nathalia Saraiva Morais, Eva Gomes Galvão, Wesley dos Santos Santos, José Cleiton Sousa dos Fechine, Pierre Basílio Almeida Gonçalves, Luciana Rocha Barros |
| Palavras-chave: | Enzimas;Engenharia química;Lipases;Octyl-nanoparticles |
| Data do documento: | 2017 |
| Citação: | RIOS, Nathalia Saraiva.; MORAIS, Eva Gomes; GALVÃO, Wesley dos Santos; SANTOS, José Cleiton Sousa dos; FECHINE, Pierre Basílio Almeida; GONÇALVES, Luciana Rocha Barros. Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, XXI; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, XII., 3 a 6 de set. 2017, Aracaju, Sergipe, Brasil. Anais [...] Aracaju, Sergipe, 2017. |
| Resumo: | In this study, lipase from Pseudomonas fluorescens was immobilized on octyl-nanoparticles by hydrophobic interaction, producing the biocatalyst OCTYL-NANO-PSF. In order to stabilize the immobilized enzyme on OCTYL-NANO, an additional step was conducted, which is the reticulation with different concentrations of glutaraldehyde (GA), producing OCTYL-NANO-PSF-GA preparations. The concentrations of glutaraldehyde were 0.5, 1, 2.5 and 5 % v/v in sodium phosphate buffer, pH 7, 200 mM. The results show that the PSF could be immobilized on octyl-nanoparticles, events at short contact time between enzyme and support (Yield of immobilization about 75 %). The reticulation step was also shown successful, since the biocatalysts remain active (Activity retention above 55 %) and it was much more stable than OCTYL-NANO-PSF preparation. Best results of activity and stability was achieved when 5% was used (OCTYL-NANO-PSF-GA 5 %) for the reticulation. This biocatalyst was 65 fold more stable than adsorbed preparation (OCTYL-NANO-PSF). |
| URI: | http://www.repositorio.ufc.br/handle/riufc/54436 |
| ISSN: | 2447 2816 |
| Aparece nas coleções: | DEQ - Trabalhos apresentados em eventos |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| 2017_eve_nsrios.pdf | 795,5 kB | Adobe PDF | Visualizar/Abrir |
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