Avaliação da variabilidade genética do vírus da infecção hipodermal e necrose hematopoiética (IHHNV) no Camarão Rosa, Farfantepenaeus subtilis, infectado experimentalmente

Abstract

Viruses are the pathologies that cause greater impact in the shrimp farming and consequential biggest economic damages registered in the sector. Despite Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is considered a pathogen of low impact in Litopenaeus vannamei species, it can bring some negative economic implications for the shrimp industry, as reduction of growth rates and low productivity. An experiment carried through in the Center of Aquatic Organims Disease Diagnosis (CEDECAM, Labomar, UFC) included the PCR detection of the IHHNV in native species of shrimps Farfantepenaeus subtilis experimentally per os infected. Results analysis showed no expected amplicons that could be generated by genetic modifications in the virus or non-specific amplifications, where the precision of the molecular diagnosis could be questioned. The present work had as objective to approach the variability of the IHHNV through the genetic sequencing of produced amplicons. These sequences were compared with those already published, showing that it does not have enough variability to compromise the results of the PCR molecular diagnosis. Additionally, the identified virus was compared with others of different origins, showing great similarity with that proceeding from the Hawaii and Equador