Resposta imune humoral de camundongos imunizados pelas vias oral e nasal com Papaya lethal yellowing virus

Abstract

The Papaya lethal yellowing vir us (PL YV), known for its capacity to naturally infect papaya ( Carica papaya L.), promoted a humoral immune response in mice immunized via oral and nasal application. ELISA and immunodiffusion tests detected specific antibodies in sera of animals orally immunized with a crude extract of infected leaves and with purified virus, and in sera of animals immunized by nasal route with purified virus. Quantification of the specific antibodies revealed predominance of synthesis of immunoglobulins-G (IgG). Immune response was not detected, with synthesis of specific antibodies, against the proteins of the uninfected leaves. Due to the immunogenicity induced by PL YV and the fact it is not pathogenic to animal or humans, this virus can be considered a candidate as a carrier and/or vector of antigens to be used in immunization procedures