Importância da utilização de ácido alfa lipóico (ALA) e catalase (CAT) no processo de criopreservação de folículos ovarianos pré-antrais, visando reduzir os danos causados pelo estresse oxidativo

Abstract

Cryopreservation of preantral ovarian follicles is a promising prospect for the preservation of oocyte fertility and oocyte development or in the case of pathologies and therapeutics that may compromise follicular reserves. The great advantage of cryopreservation of ovarian tissue is that it is independent of the age and stage of the menstrual/estral cycle, and involves fewer ethical and social issues. However, as any exposure of a biological material to extremely low temperatures, cryopreservation can lead to an imbalance in the production of reactive oxygen species (ROS), which can cause cell damage. In vivo, ROS are balanced by the antioxidant defense system, however, in vitro, the lack of physiological system can lead to oxidative stress. Therefore, antioxidants have been used before or after cryopreservation, which can be emphasized alpha lipoic acid or thioctic acid (ALA) and catalase (CAT). Therefore, the objective of this article is to help in understanding the relevance of cryopreservation of ovarian tissue for the preservation of female fertility, as well as the control of ROS production during the process, and the need for supplementation of antioxidants such as ALA and CAT.