Validações de metodologias em CLAE para análise de Azoxystrobin e Tebuconazole em produto agroquímico e nos efluentes gerados

Abstract

The ever-growing population has forced an equal growth on food production through the world. In this way, to obtain higher productivity rate on cultivated land, the use of agrochemicals spread worldwide. Having Brazil become the largest exporter of food products in the world, the use of this kind of products has increase drastically in a short time and, with that, the necessity of analytical methods to ensure the quality of these products. The cross contamination between agrochemicals products offers one of the biggest risks with quality and efficiency on the field. The cross contamination is something that must be avoid throughout all the supply chain of these products, for that, risk assessments are made and determine certain acceptable limits of contamination of an active ingredient (AI) from one product to the next one. High Performance Liquid Chromatography is the most widespread technique to ensure quality for agrochemicals. Thus, fast analytical methods, sensitive, precise and exact should be developed to determine the level of these IA. This study has the purpose to validate two analytical methods, on High Performance Liquid Chromatography coupled to a Diode Array detector, for the IA Azoxystrobin and Tebuconazole on the agrochemical product and in the samples generated during the decontamination of the production site, evaluating the required parameters according to ABNT NBR 14029:2016, which are: selectivity, linearity, accuracy and precision. The proposed methods demonstrate to be specific and selective for the AI and showed linearity in the working range of 96–144 g L-1 to Azoxystrobin and 128–192 g L-1 to Tebuconazole on the product and the range of 1 - 9 ppm to Azoxystrobin and 5 – 250 ppm to Tebuconazole for the decontamination products. The proposed methods achieved acceptable levels of recovery and precision to Azoxystrobin and Tebuconazole, demonstrating that the methods are accurate and exacts as required by the standard.