Atividade lipolítica in vitro de actinobactérias em condições abióticas extremas

Abstract

The enzymatic activity of the microorganisms in the soil contributes to the availability of nutrients, allowing the understanding of the structure and f unctioning of the microbial community and other ecosystem processes. Actinobacteria constitute a bacteri al group abundant in the soil, in which they stand out for producing important substances for the environment, as the extracellular enzymes. Its occurre nce in extreme environments is a function of adaptive mechanisms that promote the selection of strains w ith biotechnological and ecological potential. Among these enzymes the lipolytics allow the degradation of complex lipids, whose resulting products are used for the growth of other microorganisms. These enzymatic activities are affected by abiotic factors such as temperature, pH, and salinity, which can drastically influence microbial activity by limiting the capacity of their metabolic capacity. The pres ent study aims to evaluate the production of lipolytic activity in vitro and the influence of abiotic fa ctors on these activities. Analyze a lipolytic activity of bacterial activities of Parque Nacional de Ubajara (CE), a Brazilian semiarid region, where 8 4% of the tested strains are active. The samples that obtained enzymatic activity were submitted to physiological salinity, pH and temperature tests in the pH tests, as the samples show a better growth in the pH values 7 and 9, standing out as the sa mpl es UB02, UB03, UB04 and UB23, which exert lipolytic activity at all pHs. No temperature test 63.16% of the samples found no activity at the temperatures tested. The selected or potential results of active substances for lipase production and that this a cti vity is influenced by extreme clinical factors.