Avaliação da capacidade de biomodificação do colágeno de diferentes polifenóis de origem natural

Abstract

In the attempt to increase the longevity of adhesive procedures, strategies have been employed, among them we can highl ight the crossli nki ng of denti n collagen by means of d ifferent polyphenols. Therefore, the objective of the pre se nt study was to compare t he ability of crosslinking formations between ellagic acid , hesperidin , apigenin and curcumin using 20μM, 200μM and 2 m M concentrations F or th is, one hundred and thirty dentin bars were prepared with 1.7 X 0.5 X 6 mm in size, the bars were completely demineralized in 10% phosphoric acid solution for 5 hours at room temperature. The agents of biomod ification were diluted i n sodium hydroxide (NaO H ) in relevant concentration to each group with the aid of a magnetic stirrer until completely diluted, followed by filtering the solution. The dentin bars were randomly distributed in 12 distinct groups (n = 10) and kept in their re spective solutions for a period of 1 hour. Afterwards they were submitted to 3 point flexural tests, mass change and Fourier transform infrared spectroscopy (FT IR), using, respectively, a universal test machine, precision scale an d spectrometer. From the data obtained, a n o rmal ity test was performed using the Kolmogorov Smirnov test, followed by t wo way ANOVA and Tukey's post test. When the modulus of elasticity (ME) was analyzed, curcumin and 200μM ellagic acid we re effective in i n creasing ME, while hesperidin and apigenin were effective at 20μM concentration. It was observed that more than one concentration was effective in each group. No significant mass variations were observed. There were non proportional peaks in relation to th e increase of the concentration in the region 3000 and 3600cm 1 corresponding to the region that indicates the formation of hydrogen bonds. Studies should be conducted on the influence of non conventional solvents on the collagen cross linking process. Bio modification of dentin collagen is an important strategy in improving dentin matr ix properties, and polyphenols are valuable agents due to their potential for improving the mechanical properties of dentin.