Caracterização das atividades cardiorenal e neural de Bothrops marajoensis e suas frações

Abstract

In this article we evaluated the activity of Bothrops marajoensis (Bmj) and its fractions in the cardio-renal system. The results of the total venom in blood pressure experiments showed a decrease in the mean arterial pressure and heart rate without significant changes in respiratory rate. The same experiments performed in rats atropinized showed the permanence of falling blood pressure and heart rate. After administration of Bmj used in infusion of isolated rats heart of there was a decrease in myocardial force of contraction accompanied by an increase in perfusion pressure, without changes in coronary flow. The electrocardiographic analysis after injection of Bmj in rats causes a progressive atrioventricular block until a complete blockage and difficulty in atrial conduction. The assessment in the mesenteric vascular bed Bmj did not produce significant changes. The system renal perfusion in rats caused significant decrease in perfusion pressure, renal vascular resistance, urinary flow, filtration rate, transport of sodium and chloride. The phospholipase (PLA2) type 1 showed only an alteration in the transport of electrolytes. The Bmj fractions neurotoxicity in rat phrenic nerve diaphragm increasing showed a blockage dose-dependent in the strength of contraction. In mice vas deferens we observed an induced of a dose-dependent inhibition of contraction stimulated by electric field. This fact was not reversed by yohimbine or by naloxone. In another study the addition of the total venom of Bmj inhibited the neurogenic contraction, compared with no significant decrease in contraction by Cch, NA or ATP (in normal Krebs solution or with enriched with guanethidine and phentolamine). The fractions, PLA2 (type 1 and type 2) showed a dose-dependent inhibition of contraction.