Estudo do potencial químico de Fusarium udum

Abstract

The present study aims to investigate the chemical potential of Fusarium udum, an endophytic fungus isolated from Chrotalaria paulinea, which is responsible for causing damage to bean planting and species of Chrotalaria. The fungus was cultivated in four distinct culture media: PD (potato-dextrose), PDY (potato-dextrose-yeast), MPD (malt-peptone-dextrose), and Czapek. The culture time was determined by the glucose rate of the culture medium. The ethyl acetate extract from the microorganism cultivated in MPD (25 days) was selected for the pre-liminary chromatographic treatment, and led to the isolation of tyrosol, fusaric acid, and monaspilosin. In another experiment that used the culture media mentioned above, F. udum produced secondary metabolites through different days of incubation (7, 14, 21, 28 days). The chromatographic fractionation of the ethyl acetate extract from the microorganism cultured in PDY (28 days) provided cycle (L-Pro-L-Leu) and triptophol. The micellar extract obtained in this culture was also studied and it supplied the substance adenosine. Usual chromatographic techniques, including liquid-liquid partition, C-18 cartridge chromatography, and high perfor-mance liquid chromatography (HPLC), were used to isolate the secondary metabolites. The structural characterization was conducted by using the spectrometric techniques mass spec-trometry (MS) and nuclear magnetic resonance (NMR) with one and two-dimensional experi-ments and comparison with literature data.