Efeitos do fator de crescimento e diferenciação-9 e do hormônio folículo estimulante sobre o desenvolvimento in vitro de folículos pré-antrais bovinos

Abstract

The results show that after 6 days of culture, FSH alone or associated with GDF-9 increased follicular diameter in relation to control medium. Moreover, after 12 days of culture, FSH promoted an increase in follicular diameter, while the association of FSH with GDF-9 significantly reduced follicular diameter when compared with follicles cultured in MEM plus FSH. Furthermore, FSH and GDF-9 increased the antrum formation after 12 days of culture (P<0.05). Despite GDF-9 had significantly reduced the levels of mRNA for HAS 1 when compared to MEM, this factor has increased the levels of versican and perlecan. In addition, the presence of both FSH and GDF-9 increased mRNA levels for HAS 2, but reduced level those for PCNA. In addition, FSH also acts by reducing mRNA levels for PCNA. In conclusion, FSH and/or GDF-9 promotes the follicular growth and antrum formation, and GDF-9 stimulates the expression of versican and perlecan and interact positively with FSH to the increased expression of HAS 2