Avaliação do potencial de biodegradação de gasolina por bactérias do gênero burkholderia

Abstract

The generic taxon Burkholderia presents a high metabolic diversity that allows these protobacteria live in a wide range of habitats, among these are the soil, water (including sea water), plants, fungi, animals and even humans. A remarkable biotechnological application is the capacity to promote the pollutants biodegradation. Thus, this study aimed the identification of Burkhoderia SMF 090 isolate, as well the evaluation of its potential on the utilization of gasoline as a carbon source and the identification of metabolic pathways possibly involved in the degradation of gasoline on its components through realization of proteomic analysis. The identification of the SMF 090 isolate and the analysis of molecular phylogeny were carried out by 16S rRNA gene sequencing. In addition, the molecular phylogeny was analyzed and phylogenetic trees were reconstructed using other bacteria from the same genus. In order to characterize the growth pattern of SMF 090, bacterial growth curves were assessed using the media TY (a nutritive media), BH (a medium without carbon), BH plus commercial gasoline and BH plus D-glucose. The study of differentially expressed proteins was performed using bidimensional electrophoresis, mass spectrometry and bioinformatics analysis. After the 16S rRNA analysis was suggested that SMF 090 is related to Burkholderia sabiae Br3407 and both descended from a recent common ancestral. The strain grown in mineral media containing gasoline as the unique carbon source. Differentially expressed proteins were observed between the tested groups and these proteins may be associated to the metabolic degradation of hydrocarbons. The analysis of data provided by 2DE electrophoresis and mass spectrometry allowed the identification of various proteins related to the monocyclic and polycyclic aromatic hydrocarbons catabolism pathway. Therefore, this study showed relevant results about the growth of Burkholderia as a potential gasoline biodegradant.