Estudos de Cultura de Tecidos, In Vitro, de Macroalgas Marinhas da Espécie Gracilaria birdiae

Abstract

Seaweed consists in a vital resource to the economy of many countries and it has been explored all over the world thanks to its capacity of producing colloids such as agar, carrageen and sodium alginate as well as the useful aspect either in food industry, fertilizers or medicine area. Studies on induction, cultivation and callus reorganization have been shown an important issue on tissue culture technique and its usage. In different tissue experience in vitro the usage of regulating growth is so fundamental to establish the competence and necessary conditions to callus formation and regeneration. In this present study has been tested the auxin and citocinin effects in different dosages of agar in ASP 12-NTA. The indolacetic acid effect and 6- benzilalaminopurine have been tested as well in seaweed in the species Gracilaria birdiae with two separated doses, 0,5mg/L and 0,8mg/L and together in concentration of 1:5mg/L and 5;1mg/L. Regarding to agar concentration, it has been tested two concentrations with 0,5% and 0,8% in all treatments. As to sterilization process has been done with fungicide (nistatina) and antibiotic (ciprofloxacin) besides iodopovidona and the sodium hypochlorite. The complete experiment had been lead in a germination chamber with 25±2ºC and photoperiod of more or less 16 hs of light and 08hs dark and 30,2‰ saltiness, pH around 7,6 within 60 days approximately. In more or less 50 days of cultivation had been noticed callus formation and regeneration process therein. The results had been underwent by statistic analyze treatments of contingent through (x2) where many meaningful different observations had been checked in callus and regeneration and the agar levels studied, that is to say the agar level treatments in 0,8% presented a major number of regeneration