http://repositorio.ufc.br/handle/riufc/21995 Wed, 08 Apr 2026 21:35:49 GMT 2026-04-08T21:35:49Z http://repositorio.ufc.br/handle/riufc/85705 Título: Análise do crescimento da microalga Chlorella vulgaris em meios de cultura distintos Autor(es): Aquino, Yasmine Maria Lima de Oliveira Abstract: Microalgae, photosynthetic microscopic organisms, have attracted much attention from scientists due to its biotechnological potential, because its biomass can be explored as source of biofuels, and also, substances with high nutritional and commercial value, like proteins, fatty acids ans vitamins. On this study, the microalgae Chlorella vulgaris was utilized. A preliminary assay was performed on non-aerated conditions, where three different formulations of the inorganic fertilizer NPK (nitrogen:phosphorus:potassium) 19:19:19, 16:08:32 and 12:43:12 were compared. The culture which grew in NPK 16:08:32 medium showed the best results (higher cellular densities), being chosen to be compared with WC synthetic medium on aerated conditions. When the cultures cultivated on aerated conditions, with the different medium (WC and NPK 16:08:32), were analyzed, a better microalgae growth was observer with the inorganic fertilizer. Moreover, the microalgae was also cultivated in an open system, with the utilization of inorganic fertilizer (NPK 19:19:19), which showed acceptable growth during the cultivation time, enabling the use of the obtained biomass as inoculum to higher scale cultivation, as well as extraction of high value compounds produced by this microalgae. Tipo: TCC Wed, 01 Jan 2014 00:00:00 GMT http://repositorio.ufc.br/handle/riufc/85705 2014-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/85704 Título: Aspectos estruturais do reconhecimento de carboidratos por uma lectina manose-específica de sementes de Dolichos lablab com atividade pró-inflamatória e hipernociceptiva Autor(es): Osterne, Vinícius José da Silva Abstract: Lectins are proteins that specifically bind to carbohydrates and form complexes with molecules and biological structures containing saccharides, without altering the covalent structure of the glycosyl ligands, these proteins have great biotechnological potential because of its diverse biological activities. The aim of this study was to investigate the effects of Dolichos lablab lectin in inflamatory process and analyze its interaction with carbohydrates. The Man/Glc-specific lectin of Dolichos lablab, termed FRIL, is a dimeric two-chain (αβ)2 lectin presenting 67 kDa molecular mass. The interaction of FRIL with carbohydrates, as determined by molecular docking, showed that this lectin has highest affinity to the carbohydrate trimannoside (-135.618 MDS), followed by trehalosamine (-127.072 MDS) and αα-trehalose (-121.729 MDS). FRIL evoked dose-dependent paw edema, increasing animal paw volumes. The edematogenic effect of FRIL was paralleled by a significant increase in vascular permeability, about 10-fold higher compared to control. FRIL also significantly raised the animals flinch reaction in the first, third and fifth hours in response to mechanical stimulation. Injection of α-methyl-D-mannoside associated with FRIL inhibited either edema, at the third hour and hypernociception. The histopathological analysis of animal paws showed a characteristically acute inflammatory process that included severe infiltration of mixed leukocytes, changes in cytoarchitecture, edema and focal areas of hemorrhage. In addition, in silico assays confirmed that FRIL preferentially interacts with trimannoside that makes up the core N-glycans cell. Therefore, our study supports the hypothesis that the lectin domain and/or the likely glycoconjugates containing α-methyl-D-mannoside and trimannosides residues contribute to the inflammatory effects of FRIL. Tipo: TCC Tue, 01 Jan 2013 00:00:00 GMT http://repositorio.ufc.br/handle/riufc/85704 2013-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/85703 Título: Correlação entre patentes depositadas e publicações de artigos científicos sobre proteases vegetais aplicadas na fabricação de queijo e leite hipoalergênico Autor(es): Albano, Roberta Laiz Bezerra Santos Abstract: The main advantage of using enzymes as biotechnological inputs is the possibility to make industrial processes in a moderate condition, mainly because enzymes are specific to their substrate. Proteases are enzymes widely used in food processing. Proteases are pivotal tools in the manufacture of cheese, as a coagulating agent, and in the production of hypoallergenic milk, as a hydrolization agent of proteins that may be responsible for the onset of allergies. This study aimed at making a correlation between the number of patents and published scientific papers related to plants or latex proteases used in the production of cheese and hypoallergenic milk worldwide since 1976. There is an increasing interest in new proteases suitable for milk processing even due the fall in the slaughter of calves, causing shortness of rennet and hence an increase in its cost. The searches for patents and scientific publications were conducted using the keywords “plant”, “latex”, “protease”, “peptidase”, “proteinase”, “milk”, “dairy”, “casein” and “cheese”, combined through the operators " AND" and " OR ". Patents were searched on the international databases USPTO (United States), Derwent Innovations Index (global) and SPACENET-Worldwide (European database with worldwide patents) and the INPI for national patents deposits. For the papers research on the subject it was used the Web of Science portal. As a result it was only found four patents, none of which referred to protease latex, however the number of published scientific articles on studies of plant proteases applied to the production of cheese and milk hydrolysates has been growing over the years, specially from the 1990s. There is a clear mismatch between the number of published scientific studies and the number of patents applied for this purpose. This shows that prospecting for biotechnological potential is intense. However there is no obvious investment/entrepreneurship to transform potential into commercial products and thus stimulate patent applications. One of the reasons for the few patent applications is that scientists need to publish articles in order to have a better recognition and, consequently, a higher rate of projects approval by funding agencies. Once the research results are public, it is no longer possible to patent it. Tipo: TCC Tue, 01 Jan 2013 00:00:00 GMT http://repositorio.ufc.br/handle/riufc/85703 2013-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/85702 Título: Avaliação do potencial de Moringa oleifera L. na fitorremediação de águas contaminadas pelo herbicida atrazina Autor(es): Forte, Mayara Melo Abstract: Phytoremediation is a low-cost emerging technique, which uses plants to remove, transform or stabilize soil, air and water contaminants. Some plants, counted as good candidates for soil and water remediation, have been evaluated regarding the genetics and biochemistry processes involved in the capitation, transport and storage of the pollutants. Moringa oleifera presents some of the important characteristics in the selection of plant species for phytoremediation. Among them, stand out its coagulating/floculating properties, good adaptability to Ceará state's climatic conditions, fast growth rate and high resistance to diseases. Thus, the present work aims to evaluate the potential of M. oleifera for the phytoremediation of water contaminated by atrazine, an herbicide widely used in the world. For this, M. oleifera plants were put in contact with water artificially contaminated by atrazine in the 0.2, 1, 2 and 20 mg/L concentrations. The phytoremediation potential was evaluated through vegetal growth parameters, enzymatic activities and the capacity to reduce the atrazine concentration in water samples. The results demonstrate that the plants were intolerant to the 2 and 20 mg/L atrazine dosages. However, the plants submitted to the 0.2 and 1 mg/L dosages could survive until the end of the experiment, even though they presented evidence of phytotoxicity. The measure of enzymatic activity related to oxidative stress proved that treated plant's leaves and roots presented distinct profile of molecules. Overall, were observed a significant increase of peroxidase enzymes (POX and APX), especially in roots of plants submitted to atrazine treatment. The evaluation of the levels of atrazine in the hydroponic medium showed that within two days of treatment, the moringa plants reduced atrazine levels by 43%. After 10 days of treatment, the degradation rate was 89%. The results indicate that M. oleifera is a species with great potential for phytoremediation of environments contaminated with atrazine and other compounds of environmental relevance. Tipo: TCC Fri, 01 Jan 2016 00:00:00 GMT http://repositorio.ufc.br/handle/riufc/85702 2016-01-01T00:00:00Z