http://repositorio.ufc.br/handle/riufc/392 2026-06-17T02:19:21Z http://repositorio.ufc.br/handle/riufc/49890 Título: O uso de linhagens leucêmicas e a sua importância na oncologia experimental Autor(es): Sales, Lívia de Oliveira; Nogueira, Beatriz Maria Dias; Silva, Emerson Lucena da; Moraes, Maria Elisabete Amaral de; Montenegro, Raquel Carvalho; Moreira-Nunes, Caroline de Fátima Aquino Abstract: Experimental oncology is based on a model of study through cell culture, a technique frequently used for the study of cancer biology and for tests of new anticancer therapies. This model provides an appropriate system for researching the effects of drugs and toxic compounds on cells, mutagenesis and carcinogenesis, in drug screening and development. In addition the cell lines allows consistent and reproducible results, becoming one of the main tools used in experimental oncology, with the advantage of being derived from patients and easily manipulated in the laboratory. The use of cell lines is fundamental in the research related to the study of new antineoplastic drugs for the treatment of patients with chronic myeloid leukemia (CML) who are still refractory to the currently available therapies. Thereby, the understanding of the mechanisms of action and resistance and sensitivity patterns of these chemotherapies through the use of these lineages can make cancer therapy more efficient and specific. Thus, we highlight in this chapter the applications of cell lines as a study model for the various biological aspects of cancer in Experimental Oncology. Tipo: Capítulo de Livro 2019-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/49888 Título: Análise in silico da farmacocinética e farmacodinâmica do composto benzotiazólico com potencial antitumoral contra linhagem de adenocarcinoma gástrico Autor(es): Mesquita, Felipe Pantoja; Lima, Luina Benevides; Daniel, Julio Paulino; Portilho, Adrhyann Jullyanne de Sousa; Oliveira, Lais Lacerda Brasil de; Silva, Emerson Lucena da; Chazin, liza de Lucas Chazin; Vasconcelos, Thatyana Rocha Alves; Moraes, Maria Elisabete Amaral de; Montenegro, Raquel Carvalho Abstract: Gastric adenocarcinoma has high incidence and mortality rates worldwide. The aim of this study was to evaluate the antitumor activity of benzothiazole AFN01 against ACP03 gastric adenocarcinoma cell line. Furthermore, computational prediction of the pharmacokinetics and pharmacodynamics of this molecule and the molecular docking with the relevant pharmacological target found were performed. The MTT assay was performed to evaluate the cytotoxic potential of the compound and fluorescence microscopy was used to measure cell death induction with Fluorescein Diacetate, Hoechst 33342 and Propidium Iodide fluorophores. The result of the concentration-response curve obtained by MTT assay revealed a relevant cytotoxic effect against the lineage with IC50 values around 17.24 μM at 24 h, 8.23 μM at 48 h and 1.39 μM at 72 h of exposure. AFN01 was able to induce apoptosis at 1 and 2 μM. Computational analyses identified relevant pharmacokinetic properties such as positive intestinal absorption, inhibition of CYP1A2, CYP2C9 and CYP3A4, as well as weak inhibition of hERG and class III acute oral toxicity. The pharmacodynamic prediction identified molecular targets related to antitumor activity. The enzyme TDP1 was selected for molecular docking, indicating that benzothiazole has a binding energy of -7.5 kcal/mol and important interactions with catalytic site residues of the enzyme. In conclusion, the AFN01 benzothiazole compound is an excellent candidate for the treatment of gastric adenocarcinoma, with good predicted pharmacokinetic parameters and a potentially new molecular target. Tipo: Capítulo de Livro 2019-01-01T00:00:00Z