http://repositorio.ufc.br/handle/riufc/22841 2026-04-06T00:03:35Z http://repositorio.ufc.br/handle/riufc/63706 Título: Evidência de dano precoce da cartilagem na osteoartrite experimental por meio de microscopia eletrônica de varredura Autor(es): Girão-Carmona, Virgínia Cláudia Carneiro; Bezerra, Mirna Marques; Porto, Fernanda Maria Aragão Ximenes; Pinto, Ana Carolina Matias Dinelly; Nunes, Rodolfo de Melo; Alves, Anelise Maria Costa Vasconcelos; Campello, Claudio Cabral; Leite, Ana Caroline Rocha de Melo; Brito, Gerly Anne de Castro; Rocha, Francisco Airton Castro da Abstract: Images obtained by scanning electron microscopy of articular cartilage samples from mice submitted to an experimental model of osteoarthritis for semiquantitative analysis. Tipo: Artigo de Periódico 2022-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/63276 Título: Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities Autor(es): Pacífico, Dvison de Melo; Costa, Cecília Leite; Moura, Hercules; Barr, John R.; Maia, Guilherme Augusto; Benetti Filho, Vilmar; Moreira, Renato Simões; Wagner, Glauber; Domingues, Regina Maria Cavalcanti Pilotto; Quesada-Gómez, Carlos; Ferreira, Eliane de Oliveira; Brito, Gerly Anne de Castro Abstract: Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC‑45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high‑performance liquid chromatography‑tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC‑45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC‑45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC‑45 strain; 14 proteins, including mature S‑layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains. Tipo: Artigo de Periódico 2021-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/63273 Título: The effect of high concentration of zoledronic acid on tooth induced movement and its repercussion on root, periodontal ligament and alveolar bone tissues in rats Autor(es): Sousa, Fátima Regina Nunes de; Ferreira, Vanessa Costa de Sousa; Martins, Conceição da Silva; Dantas, Hugo Victor; Sousa, Frederico Barbosa de; Girão-Carmona, Virgínia Cláudia Carneiro; Goes, Paula; Brito, Gerly Anne de Castro; Leitão, Renata Ferreira de Carvalho Abstract: Zoledronic acid (ZA) is often prescribed for osteoporosis or resorptive metabolic bone disease. This study aims to evaluate the effect of ZA on orthodontic tooth movement (OTM) and root and bone resorption and its repercussion on root, periodontal ligament and alveolar bone tissues. The experimental group consisted of 72 Wistar rats divided in four subgroups: Naive, Saline and Zoledronic Acid groups at the concentration of 0.2 mg/kg [ZA (0.2)] or 1.0 mg/kg [ZA (1.0)]. The animals were subjected to i.v (dorsal penile vein) administrations of ZA or saline solution, on days 0, 7, 14 and 42. Under anesthesia, NiTi springs were installed in the first left maxillary molar with 50gf allowing the OTM, except for the negative control group (N) for mesial movement of the left first maxillary teeth. The animals were sacrificed and maxillae were removed for macroscopic and histopathological analyzes, scanning electron microscopy, computerized microtomography and confocal microscopy. Treatment with ZA decreased the OTM and the number of osteoclasts and loss of alveolar bone when compared to the naive and saline groups. Reduction of radicular resorption, increased necrotic areas and reduced vascularization in the periodontal ligament were observed in the ZA groups. ZA interferes with OTM and presents anti-resorptive effects on bone and dental tissues associated with a decreased vascularization, without osteonecrosis. Tipo: Artigo de Periódico 2021-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/63272 Título: Analysis of the immunoexpression of opioid receptors and their correlation with markers of angiogenesis, cell proliferation and apoptosis in breast cancer Autor(es): Sousa, Alceu Machado de; Dantas, Thinali Sousa; Silva, Paulo Goberlânio de Barros; Martins, Conceição da Silva; Freire, Gildenio Estevam; Ribeiro Junior, Howard Lopes; Brito, Gerly Anne de Castro; Pereira, Karuza Maria Alves; Leitão, Renata Ferreira de Carvalho Abstract: Objective: Breast cancer is a disease of great concern. The prognosis of this tumor is related to its staging. Opioids are widely used to minimize pain in oncology clinics; however, the relationship between the administration of opioids and their effects on tumor cells has yet to be elucidated. Therefore, this study aimed to evaluate the immunoexpression of mu- (μ) and kappa- (κ) opioid receptors and their correlation with markers of angiogenesis, cell proliferation, and apoptosis in biopsies of breast tumors. Methods: Demographic data, tumor characteristics, opioid use, and prognostic factors were collected from medical records. After the selection of the excisional biopsies, immunohistochemistry was performed for μ- and κ-opioid receptors, vascular endothelial growth factor (VEGF), Ki-67, and TUNEL. Results: A significant predominance of Ki-67 and μ-opioid receptor immunoexpression in the lymph nodes was observed in patients administered opioid medications. The luminal A subtype showed higher apoptosis levels (TUNEL) compared to the luminal B subtype. Patients with T4 tumor who had recurrence demonstrated a reduced expression of κ-opioid receptors at the lymph node location. Correlation analyses between the μ and κ opioid markers, VEGF, Ki-67, and TUNEL showed that these findings are likely involved in the same mechanisms the cancer of T4 stage breast cancer. Conclusion: The κ-opioid receptor has a lower immunoexpression in nodal tumor metastasis with recurrence, whereas the μ-opioid receptor is directly related to expression of TUNEL-positive cells in tumors and indirectly to Ki-67 in nodal metastasis. Neither of the two receptors was expressed in the primary tumor or nodal metastasis in relation to VEGF. Tipo: Artigo de Periódico 2021-01-01T00:00:00Z