http://repositorio.ufc.br/handle/riufc/22105 2026-04-02T14:48:39Z http://repositorio.ufc.br/handle/riufc/82304 Título: Comunidade zooplanctônica da água da Praia de Barreira, Icapuí/CE Autor(es): Pereira Junior, Antônio Monte Abstract: Zooplankton are an essential component of marine ecosystems, important for the transfer of energy between primary producers and higher trophic levels, exerting great ecological, biogeochemical and socioeconomic impact. This study aimed to analyze the zooplankton community present at Barreiras Beach in Icapuí/CE, as well as to verify the presence of bioindicators of water quality and to analyze the salinity, pH, transparency, temperature, dissolved oxygen, phosphate and ammonia levels in the beach water. Monthly collections were carried out between July and December 2024 and January 2025, always at low tide, with 100 liters of water filtered with a 25 μm mesh net and 25 cm mouth diameter. The collected material was concentrated to 10 mL, fixed in 4% formalin in a 1:1 ratio, and 10 subsamples of 0.1 mL were analyzed under a Callmex® microscope. In situ data were obtained on water temperature and transparency, salinity, pH, dissolved oxygen, ammonia and phosphate levels. The results revealed ammonia levels, with an average value of 1,300 mg.L-1 and phosphate, with an average value of 0.102 mg.L-1, above ideal levels, which together with the trophic state index, TSI (PSR) 59 and TSI (DS) 56, showed an environment under strong environmental impact. Regarding the zooplankton community, it was composed of four main groups of organisms: rotifers, copepods, protozoa and crustacean larvae, with emphasis on the dominance of Keratella sp., and the presence of Brachionus sp., Vorticella sp., Centropyxis aculeata e Filinia terminalis, as bioindicators of eutrophication and/or pollution conditions. These results reveal the need for continuous monitoring of the beach, with the aim of evaluating the evolution of environmental quality and assisting in the implementation of public policies to minimize the impacts of anthropogenic actions and preserve the water quality of Barreira Beach, Icapuí/CE. Tipo: TCC 2025-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/82301 Título: Proposta de análise diagnóstica de Vibrio em camarão Penaeus vannamei por PCR em tempo real Autor(es): Oliveira, Ádila Holanda de Abstract: The intensification of shrimp farming has led to a rise in bacterial diseases, particularly those caused by the Vibrio genus. Among them, Vibrio parahaemolyticus stands out as the etiological agent of Acute Hepatopancreatic Necrosis Disease (AHPND), responsible for high mortality rates in Penaeus vannamei. Given the need for rapid and sensitive diagnostic methods, this study aimed to develop and validate a real-time PCR (qPCR) protocol for the detection and quantification of Vibrio sp. and V. parahaemolyticus. Primers were selected through in silico analysis using the Primer-BLAST tool and validated through sequence alignment with MEGA software. The analysis showed that the primers for V. parahaemolyticus exhibited high specificity, amplifying 229 sequences exclusively from the target species. In vitro assays were conducted with different bacterial concentrations mixed with P. vannamei tissue for DNA extraction and subsequent qPCR analysis. The results demonstrated consistent amplification and melting curves, with high reproducibility and sensitivity. Statistical analysis revealed significant differences between experimental groups, confirming the protocol’s ability to quantify bacterial load variation. Thus, the developed protocol proved to be efficient for the molecular diagnosis of Vibrio sp. and V. parahaemolyticus, representing a promising tool for health monitoring in aquaculture Tipo: TCC 2025-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/81882 Título: Aquicultura 4.0: Sistema de gerenciamento de fazenda Autor(es): Santos, Pedro Roger Alves dos Abstract: Aquaculture is one of the fastest-growing animal production sectors worldwide, playing a key role in global food security. However, the sector's growth presents challenges related to production management, sustainability, and the adoption of accessible technologies. In this context, this work proposes the development of a digital management system for aquaculture farms, aligned with the principles of Aquaculture 4.0. The main goal is to provide a free, open- source tool tailored to the realities of small and medium-sized producers, promoting greater efficiency and autonomy in the farming of tilapia and shrimp. The research was based on the analysis of four management systems currently available on the market, which revealed limitations such as high costs, low usability, and lack of adaptation to field conditions. To address these gaps, a system was designed with a focus on accessibility, mobile usability, and offline data recording, using technologies such as React, TypeScript, and modern graphic libraries. The design followed a Mobile-First approach, with prototyping carried out in Figma. The system includes modules for zootechnical planning, biometric monitoring with graphical growth visualization, a dynamic feeding table, and team management. Data is stored locally, allowing use even in areas without internet access. The results demonstrate that the tool can optimize farm management, facilitate production monitoring, and support decision-making, contributing to the modernization of Brazilian aquaculture. In addition to addressing current technological gaps, the system proposes future innovations such as IoT sensor integration, expansion to other species, and the implementation of analytical dashboards. Thus, the project contributes to the democratization of technology in rural areas and presents itself as a viable and sustainable alternative to strengthen the aquaculture production chain. Tipo: TCC 2025-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/81875 Título: Avaliação da produção da microalga Limnospira platensis em reatores tipo bags indoor e outdoor Autor(es): Brasil, Guilherme de Queiros Abstract: Limnospira platensis (formerly known as Spirulina or Arthrospira platensis) is a filamentous cyanobacterium with a spiral-shaped morphology, commonly classified as a microalga. Its use in human nutrition dates back to ancient civilizations, having been consumed by peoples living near alkaline lakes in Africa and Mexico. Currently, this species is widely cultivated on an industrial scale and is considered a promising alternative for diversifying aquaculture and agro-industrial production systems. Its applications include use as a dietary supplement, an ingredient in animal feed formulations, and a source of bioactive compounds of interest in the pharmaceutical, nutraceutical, and cosmetic industries. This study aimed to evaluate the production of the microalga L. platensis in bag cultivation systems under two distinct conditions: indoor and outdoor environments. The cultivation was carried out in 50 L plastic bags using the standard Jourdan culture medium at a salinity of 20. The experiment lasted 25 days, with daily monitoring of physicochemical parameters (temperature, pH, salinity, light intensity, and optical density), followed by quantification of dry biomass and chlorophyll a content. Data were analyzed using one-way ANOVA and Tukey's test (p < 0.05). The results showed that outdoor cultivation performed significantly better in terms of cell growth, biomass yield (40 mg·L⁻¹), and chlorophyll a concentration (13.573 ± 0.501 μg·L⁻¹) compared to indoor cultivation (8 mg·L⁻¹ and 3.807 ± 0.991 μg·L⁻¹, respectively). Higher light intensity and temperature in outdoor cultivation were the main factors associated with the better performance of the outdoor system. In conclusion, the use of bags as photobioreactors is feasible and promising for L. platensis production under outdoor conditions, offering a low-cost alternative for obtaining biomass with potential applications in food, aquaculture, and biotechnological industries Tipo: TCC 2025-01-01T00:00:00Z