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Identificação de alterações genéticas e sua influência prognóstica na Leucemia Linfóide Aguda Pediátrica no Brasil: uma revisão sistemática

2021-12-16T18:21:09Z

Título: Identificação de alterações genéticas e sua influência prognóstica na Leucemia Linfóide Aguda Pediátrica no Brasil: uma revisão sistemática Autor(es): Santos, Nagyla Brenna Gonçalves dos; Nogueira, Beatriz Maria Dias; Sousa, Isabelle Cerqueira; Moraes, Maria Elisabete Amaral de; Moreira-Nunes, Caroline Aquino Abstract: “Brasil” entre os anos 2009 a 2019. Os resultados indicam que a presença da alteração na expressão dos genes HDAC3, HDAC7, HADC9 e TIMP1 estão associados a taxa de sobrevida > 5 anos. Um alelo variante do gene IKZF1 conferiu maior risco de desenvolvimento de LLA-B. A baixa expressão do gene ABCG2 foi associado ao pior prognóstico e está relacionada a resposta a quimioterapia. Crianças com hiperdiploidia tem maior frequência de mutações no gene MAPK. A presença da hiperexpressão dos genes BLVRB e IGFBP7 foram relacionados a leucocitose < 50.000 mm³ no diagnóstico. Níveis elevados de MAN1A1 estão associados a LLA entre 1 e 9 anos. Os genes KRAS e NRAS quando mutados e relacionados a translocação MLL-AFF1 conferiram pior taxa de sobrevivência global. As alterações genéticas descritas nos estudos analisados nesta revisão, mostram a importância e relevância de algumas dessas alterações no prognóstico destes pacientes, reforçando a importância da investigação destas alterações como forma de possibilitar condutas clínicas e terapias-alvo dirigidas mais adequadas ao manejo clínico destes pacientes. Tipo: Artigo de Periódico

Triethylphosphinegold(I) complexes with secnidazole-derived thiosemicarbazones: cytotoxi activity against HCT-116 colorectal cancer cells under hypoxia conditions

2020-08-17T21:25:19Z

Título: Triethylphosphinegold(I) complexes with secnidazole-derived thiosemicarbazones: cytotoxi activity against HCT-116 colorectal cancer cells under hypoxia conditions Autor(es): Oliveira, Ana P. A.; Freitas, Jennifer T. J.; Diniz, Renata; Pessoa, Claudia; Maranhão, Sarah S.; Ribeiro, Juliana M.; Souza-Fagundes, Elaine M.; Beraldo, Heloisa Abstract: Triethylphosphinegold(I) complexes [Au(HL1)P(CH2CH3)3]PF6 (1), [Au(HL2)P(CH2CH3)3]PF6 (2), and [Au(HL3)P(CH2CH3)3]PF6 (3) were obtained with (E)-2-(1-(2-methyl-5-nitro-1H-imidazol-1-yl)propan-2-ylidene)hydrazinecarbothioamide (HL1), (E)-N-methyl-2-(1-(2-methyl-5-nitro-1H-imidazol-1-yl)propan-2-ylidene)hydrazinecarbothioamide (HL2), and (E)-2-(1-(2-methyl-5-nitro-1H-imidazol-1-yl)propan-2-ylidene)-N-phenylhydrazinecarbothioamide (HL3). All compounds were assayed for their cytotoxic activities against HCT-116 colorectal carcinoma cells under normoxia and hypoxia conditions and against nonmalignant HEK-293 human embryonic kidney cells under normoxia conditions. The thiosemicarbazone ligands HL1-HL3 were inactive against HCT-116 cells under hypoxia but while HL3 was inactive, HL1 and HL2 proved to be cytotoxic to both cell lineages under normoxia conditions. Complexes (1-3) and the triethylphosphinegod(I) precursor proved to be active against both cell lineages in normoxia as well as in hypoxia. While 1 and 3 revealed to be active against HEK-293 and HCT-116 cells, being approximately as active against HCT-116 cells in normoxia as under hypoxia, complex (2) proved to be more active against HCT-116 cells under hypoxia than under normoxia conditions, and more active against HCT-116 cells than against the nonmalignant HEK-293 cells, with the selectivity index, calculated as SI = IC50HEK-293/IC50HCT-116hypoxia, equal to 3.7, similar to the value obtained for the control drug tirapazamine (tirapazamine (TPZ), SI = 4). Although the compounds showed distinct cytotoxic activities, the electrochemical behaviors of HL1-HL3 were very similar, as were the behaviors of complexes (1-3). Complex (2) deserves special interest since it was significantly more active under hypoxia than under normoxia conditions. Hence, in this case, selective reduction of the nitro group in a low oxygen pressure environment, resulting in toxic reactive oxygen species (ROS) and damage to DNA or other biomolecules, might operate, while for the remaining compounds, other modes of action probably occur. Tipo: Artigo de Periódico

Jogo de cartas como estratégia para o ensino de doenças autoimunes na graduação médica

2020-05-21T12:23:44Z

Título: Jogo de cartas como estratégia para o ensino de doenças autoimunes na graduação médica Autor(es): Braga, Catarina Joelma Magalhães; Pantoja, Lydia Dayanne Maia; Bachur, Tatiana Paschoalette Rodrigues; Aragão, Gislei Frota Abstract: The experience reported in this article involved the development and application of ImmunoDAI, a card game developed by students and professors of the medical course to address the technical content of twenty autoimmune diseases (DAI). The DAI comprise numerous diseases with different clinical presentations that share a complex but common etiology represented by the immune response against autoantigens. The ImunoDAI is a collectively produced game that, as a pedagogical resource, made possible the work with the DAI through the elaboration of four letters containing information about the main causes of the disease; immunopathogenesis; signals and symptoms; diagnosis and treatment. The use of the game facilitated the fixing of contents and favored the teaching-learning process by allowing multiple interactions, promoting content learning, developing autonomy, creativity, cooperation, discussions and decision making, skills that are indispensable for future doctors. Tipo: Artigo de Periódico

A deficiência de tiamina e niacina como fator de risco para doenças neurológicas

2020-05-21T12:21:53Z

Título: A deficiência de tiamina e niacina como fator de risco para doenças neurológicas Autor(es): Oliveira, Nayrene Amorin Carvalho; Magalhães, Laryssa Alves; Matos, Maria Rosimar Teixeira; Aragão, Gislei Frota; Bachur, Tatiana Paschoalette Rodrigues Abstract: Niacin and thiamine, vitamins obtained through diet, are precursors of coenzymes of the intermediate metabolism. The objective of the present study was to review the literature on these vitamins in oxidative metabolism and its implications for neurological diseases. The methodology consisted of a bibliographic search in the Medline and Science Direct databases, using the descriptors “oxidative stress”, deficiency, “basal metabolism”, “nervous system”, “thiamine” and “niacin”. Ten articles were obtained for the production of the review and the studies showed that the deficiency of those vitamins can cause dysfunction in the central nervous system due to different alterations in oxidative metabolism. Tipo: Artigo de Periódico