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Efeito protetor do extrato hidroalcoólico de agaricus blazei murill em testículos de camundongos expostos ao quimioterápico 5-fluorouracil

2026-03-02T19:01:47Z

Título: Efeito protetor do extrato hidroalcoólico de agaricus blazei murill em testículos de camundongos expostos ao quimioterápico 5-fluorouracil Autor(es): Damasceno, Thamyres Rodrigues Abstract: The 5-Fluorouracil (5-FU) is a chemotherapeutic agent capable of inducing infertility, compromising sperm viability and testicular tissue (TT) morphology. In this context, in the search for bioactive compounds with protective effects that may mitigate these adverse outcomes, the hydroalcoholic extract (HEAb) of the fungus Agaricus blazei Murill emerges as a potential candidate due to its anti-inflammatory and antioxidant effects in other tissues. However, no studies have investigated this compound in the male reproductive system. Thus, the aim of the present study was to evaluate the protective effect of HEAb against 5-FU toxicity on sperm viability and TT morphology in Swiss mice. For this purpose, 18 mice were subjected to three treatments: (1) Control (NaCl 0.9%); (2) 5-FU 450 mg/kg/single dose; (3) 5-FU 450 mg/kg/single dose + HEAb 25 mg/kg/3 days. After three days, the animals were euthanized, and the TT were fixed for classical histology (HE), sperm viability, cell proliferation (AgNOR), quantification of MDA and GSH to assess antioxidant activity, immunostaining for MMP-2 and MMP-9, and DNA fragmentation by TUNEL assay. Animals treated with 5-FU showed morphological alterations, reduced sperm viability and proliferative capacity of spermatogonia and Sertoli cells, and increased oxidative stress compared to the control group (p < 0.0001). However, HEAb administration preserved testicular morphology and sperm viability similar to the control, increased cell proliferation compared to the 5-FU group (p < 0.001), and reduced oxidative stress compared to the other treatments (p < 0.001). Regarding immunostaining, the 5-FU group showed a significant increase in MMP-2 and MMP-9 expression compared to the control group (p < 0.001), while the group treated with co-administration of HEAb also showed increased MMP-2 expression but reduced MMP-9 expression (p < 0.001). DNA fragmentation analysis by TUNEL revealed a significant increase in apoptosis rates in germ cells of the 5-FU group compared to the control (p < 0.001), whereas the 5-FU+HEAb group showed a significant reduction in positive TUNEL labeling compared to the 5-FU group (p < 0.05). No significant differences were observed between the 5-FU+HEAb group and the control group (p > 0.05). These findings indicate that the hydroalcoholic extract of A. blazei Murill was able to mitigate the toxic effects of 5-FU on morphology, sperm viability, oxidative stress, cell proliferation, matrix remodeling, and DNA fragmentation. Tipo: Dissertação

Material autoral digital educacional em formato de livro-jogo para o ensino do corpo humano: uma abordagem inclusiva para crianças com Transtorno do Espectro Autista

2026-02-20T17:09:22Z

Título: Material autoral digital educacional em formato de livro-jogo para o ensino do corpo humano: uma abordagem inclusiva para crianças com Transtorno do Espectro Autista Autor(es): Campos, Carla de Alencar Sarmento Abstract: Science education in the early grades of elementary school is an important component in the holistic development of students, stimulating observation, curiosity, and the ongoing and active construction of knowledge. From this perspective, the use of digital technologies and Educational Digital Authoring Materials (EDMs) emerges as an inclusive and interactive pedagogical resource capable of favoring the teaching and learning process based on meaningful learning. This work was guided by the guidelines of the Brazilian National Common Curriculum Base (BNCC) for the early grades of elementary school, according to which the teaching of Natural Sciences should promote critical, logical, and creative thinking, as well as enabling a broader understanding of natural and social phenomena. The proposal of this dissertation consists of the creation of an Educational Digital Authoring Material, in the form of a game-book, developed by the researcher using the Twine software, entitled Nina the Explorer. This material addresses the teaching of the human body and sensory organs, integrating pedagogical and playful aspects, and promoting autonomy in the learning process of children with Autism Spectrum Disorder (ASD), based on Ausubel's Theory of Meaningful Learning. Therefore, this dissertation seeks to contribute to educational inclusion and the improvement of pedagogical practices in science teaching, in alignment with the guidelines of the National Common Curricular Base (BNCC) and the principles of inclusive and quality education. The resource was validated by 25 specialists in the areas of Morphofunctional Sciences, Pedagogy, and Inclusive Education, who analyzed the material individually through a semi-structured questionnaire applied via Google Forms, accompanied by an Informed Consent Form and a confidentiality agreement. The analysis of the base material, the textbook, and the curricular documents BNCC and the Ceará Reference Curricular Document were also considered. The data were analyzed descriptively and through content analysis. The evaluators concluded that, regarding content adequacy, it is well aligned with the normative documents of basic education, although some pointed out aspects that could be improved. In terms of attractiveness and engagement, the gamebook is relevant as an inclusive and motivating resource for this audience. Regarding the pedagogical aspect, it promotes meaningful learning by presenting content in a contextualized, interactive, and playful way to stimulate reflection and practical application of knowledge. In terms of accessibility, it is suitable for the audience because it integrates attractive visual stimuli, interactivity, and an organized narrative structure. Therefore, the results of this study highlight the potential of digital technologies and a MADE in the form of an interactive gamebook, as an innovative and inclusive resource in science education. Tipo: Dissertação

Potencial terapêutico do colágeno extraído da pele de tilápia® na atenuação dos danos locais induzidos por veneno de Bothrops jararacussu: um estudo em camundongos Swiss

2026-02-18T22:42:52Z

Título: Potencial terapêutico do colágeno extraído da pele de tilápia® na atenuação dos danos locais induzidos por veneno de Bothrops jararacussu: um estudo em camundongos Swiss Autor(es): Silva, Maria das Candeias Santos Abstract: Snakebite accidents are classified as a significant public health problem and have been included in the list of Neglected Tropical Diseases (NTDs) by the World Health Organization (WHO) (Silva et al., 2017). In the Americas, snakebite envenomation represents an important public health challenge, although on a smaller numerical scale compared to Afro-Asian regions. Many patients survive due to antivenom therapy; however, they are often left with permanent sequelae. In view of this reality, the World Health Organization officially included snakebite envenomation in the list of Neglected Tropical Diseases in 2017. Bothrops jararacussu belongs to the Viperidae family and is popularly known as jararacuçu; it is a highly dangerous venomous snake, standing out as the second largest venomous species in Brazil. Its venom causes extensive local damage, as it degrades components of the extracellular matrix of various tissues and the basement membranes of blood vessels. In this context, biomaterials capable of attenuating local tissue damage have been developed and investigated. Among these biomaterials, collagen extracted from tilapia skin stands out, presenting specific biochemical properties that make it a promising candidate for application in tissue repair therapies, especially in clinical contexts involving intense skin destruction. Thus, the therapeutic potential of collagen extracted from tilapia skin in attenuating the local effects induced by Bothrops jararacussu venom in Swiss mice was evaluated. The following procedures were performed: extraction and purification of tilapia skin collagen; collagen cytotoxicity testing; irritability assay using the chorioallantoic membrane (HET-CAM); in vivo experiments in Swiss mice; histological analysis using hematoxylin and eosin staining; mast cell evaluation using toluidine blue staining; and collagen fiber analysis using picrosirius red. The results demonstrated that collagen extracted from tilapia skin showed high biocompatibility and absence of cytotoxicity, in addition to stimulating cell proliferation in vitro. In the HET-CAM assay, collagen was classified as non-irritant and significantly reduced irritation induced by Bothrops jararacussu venom. In the in vivo assays, collagen significantly attenuated the formation and area of the hemorrhagic halo at all evaluated time points, although presenting a partial effect when compared to a metalloproteinase inhibitor. Histological analyses confirmed reduced hemorrhage, edema, myonecrosis, and inflammatory infiltrate in collagen-treated groups, with preservation of tissue structure. Additionally, collagen modulated the mast cell response and promoted recovery of the collagen matrix, restoring total collagen and type I collagen levels degraded by the venom. These findings indicate that tilapia skin collagen, in addition to mitigating the deleterious local effects of snake venom, exerts a protective action on injured tissue. Although further studies are necessary to evaluate the mechanisms of action, this biomaterial emerges as a promising alternative for the initial management of local effects of snakebite envenomation. Tipo: Dissertação

Educação tecnológica & metodologias ativas

2026-01-12T11:24:23Z

Título: Educação tecnológica & metodologias ativas Tipo: Livro