Prospecção tecnológica, desenvolvimento e caracterização in vitro de nanopartículas lipossomais contendo derivados de quinoxalinas como agentes antitumorais

Abstract

Modified drug release systems are pharmaceutical preparations with strong avant-garde appeal in the frontier area of knowledge. The work was carried out to perform bibliographical research, search for anteriorities, develop and characterize standard liposomal nanoparticles. The survey and the prospection were carried out in national and international banks. Keywords were chosen that could help refine the search for liposome-related technologies. In the preparation of the liposomes dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS) and cholesterol (COL) were used. The components were dissolved in chloroform / methanol solution. The solvents were evaporated and the preparation was kept in an overnight dessicator. After the preparation was resuspended, it formed an emulsion. The emulsion was subjected to the extruder to produce a similar population of liposomes. Particle size determination was performed through dynamic light scattering measurements. The particle size distribution is obtained by the polydispersity index. The measurements of the mean zeta potential of the particles were performed by the microelectrophoresis technique. For quantification of the encapsulated material, liquid chromatography coupled to mass spectrometry (UPLC-QToF-MSE) was used. It has been found that there is not a clear correlation between published articles and deposited patents, although there are many more published articles than patents deposited and that Brazil still has inconsistent production on articles and patents on liposomes. The characterization showed monodisperse populations of small particles of 185.46 ± 3.76 nm, with a polydispersity index of 0.48 ± 0.01. The liposomes remained with an average size of 200 nm. The zeta potential was - 40.9 ± 0.96 mV, representing the residual negative charge on the surface of the particles. The results obtained in the atomic force and scanning microscopy corroborated the previous tests to verify the size of the liposome and allowed to verify its spherical shape. The consistency of the results obtained from the established method endorses its effectiveness and effectiveness. The lipophilic nature and size of the liposomes obtained appear in the same size scale of the biological macromolecules - nucleic acids, proteins, etc. - allow an integration between nanotechnology and biology, leading to advances in the use of these as drug delivery vehicles for passive strategies and active selectivity.