Fibrohistiocitoma maligno transplantável em ratos Wistat: caracterização (histopatologia, ultraestrutura, citoquimica enzimática e imunohistoquímica): estudo cinético e aspectos farmacológicos

Abstract

A spontaneous tumor arised on the ragion of salivar gland C-TEGS-2047) of a Wistar rat and transplanted with trocarter was studied under several aspects: (a) histologycal characterization Chistopathology, ultrastructure, enzimatic cytochemistry and immunohistochemistry); Cb) metastitic potential; C?) stathnokinetic study using .. Vincristine; (d) Hormonal sensitivity and C-e) search for bioactive substances produced by the tumor. The results showed: Qa) Optical and eletron microscopy studiesCCM and EM) revealed that the tumor is mainly constituted by histiocytic-like cells and also fibroblastic-like cells and undifferentiated celss.The enzymatic cytochemistry was strongly positive —for an a-naphthyl acetate esterase and acid phosphatase, suggesting the histiocytic nature of this neoplasm. Immunohistochemistry study using histiocytic (Lisozyme, a-1-Antitrypsin and a-1 Antichymotrypsin)and intermediate-sized filaments (Vimentin) markers showed activity, only for Lisozyme and • Vimentin confirming the fibrohistiocytic. nature of the tumor and suggesting that it may be considered as a malignant fibrous histipcytoma CMFH).