Efeitos renais promovidos pelo veneno total da serpente Bothrops pauloensis

Abstract

Snake envenoming is an important health problem widespread in tropical countries. According to the Department of Health of Brazil, the genus Bothrops are the main involved in snakebites in the country and acute renal failure (ARF) is a serious complication of snake poisoning. This study was investigate the effects of the Bothrops pauloensis venom(vBp) in the renal perfusion system and in cultured renal tubular cells of the type MDCK(MardinDarby canine kidney). Isolated kidney from Wistar rats weinghing 250 to 300g. were perfused with Krebs-Henseleit solution containing 6%w/v of albumin serum albumin previously dialyzed. The effects of Bothrops pauloensis venom (10μg/mL) were studied on the Perfusion Pressure (PP), Renal Vascular Resistance (RVR), Urinary Flow (UF), Glomerular Filtration Rate (GFR), Percentage of Sodium (%TNa+), Potassium (%TK+) and Chloride (%TCl-) Tubular Transport. The treatment with vBp caused decrease in cell viability to the lowest concentration tested with an IC50 of 4,18 μg/mL. B. pauloensis venom (10 μg/mL) reduction the PP and RVR at 90 and 120 min. The UF increased at 120 min. The GFR decreased at 60 min. It was also observed a decrease on percentual tubular transport of sodium (%TNa+) , of chloride (%TCl-) and potassium(%TK+) at 60, 90 and 120 min. Histological analysis of kidneys perfused with vBp showed the presence of significant morphological changes such as accumulation of proteins in tubular and glomerular spaces.