A influência do tempo de estocagem na análise de parâmetros hematológicos em processo automatizado do hemograma humano

Abstract

The CBC is, among all tests required by doctors of various specialties, as requested. Upon completion of the collection, the laboratories must follow specific procedures for storage of whole blood to preserve the cellular characteristics. Thus, this study aims to evaluate the influence of storage time on the analysis of hematological parameters in automated process in human blood count, setting a maximum time interval between collection and sample processing, to obtain reliable results. We conducted a retrospective study of blood samples from 57 adult patients of both sexes, collected by venipuncture in EDTA treated at the General Hospital of Fortaleza. The samples were kept under refrigeration 2 ° to 8 ° C and analyzed with an automatic counter Cell Dyn Ruby, at intervals of 1, 24, 48, 72 and 96 hours after collection. The results were tabulated in the following computer programs: Microsoft Word 2007, Microsoft Excel 2007, SPSS version 19 and Rgui 3.0.0. For statistical analysis we used the Friedman test with a confidence interval of 95% (p <0,05). The results were presented in tables and graphs. Parameters analyzed in our study, the first 24 hours, the hemoglobin, the hematocrit, the RBC indices (MCV, MCH, MCHC and RDW) the Global Leukocyte Count were the first to be changed. Thus, we observe that the parameters of blood count performed from peripheral blood samples, collected by venipuncture into tubes containing EDTA, stored under-cooling of 2 ° to 8 ° C, can be measured in unit Cell Dyn Ruby within 48 hours after collection, with no significant change in the values of Global Leukocyte Count, Red Blood Cells, Hemoglobin, Hematocrit, Platelets and RBC indices.