Padronização do uso do plugin Seeds Analysis no software ImageJ para avaliação de vigor de sementes e plântulas de soja (Glycine max)

Abstract

Vigor tests are used to evaluate the physiological quality of seeds. However, although these methods are extremely important for evaluating seed quality, they are performed manually, which is time-consuming and laborious. A possible alternative is to perform the evaluations using the image analysis method associated with the use of software. Thus, this study aimed to standardize the use of the SeedsAnalyser plugin, present in the ImageJ software, for evaluating soybean seeds and seedlings (Glycine max). Traditional tests were performed to evaluate vigor and images were obtained using an HP Scanjet 2004 flatbed scanner, adapted in an inverted manner, inside an aluminum box. Parameters related to morphometric characteristics available in the plugin were used to evaluate the images. With the plugin, it was possible to obtain all the data of the parameters studied for seed and seedling, and these same data correlated positively with the data obtained through vigor tests. Thus, with the use of the plugin, it was possible to accurately assess the quality of the seed lots evaluated.