Viabilidade de sêmen caprino refrigerado a 5° C diluido em soro fisiológico adicionado de clara de ovo

Abstract

The aim of this study was to evaluate the efficiency of using an extender containing egg white and saline solution in semen cooling process of native bucks. To this end, three goats were used, evaluated according to clinical andrological examination, vaccinated and dewormed, housed in individual stalls (9 m²), fed with Tifton hay and concentrate (NRC, 2007), having access to water and mineral salt ad libitum, reared at Fortaleza-CE. The semen was collected by artificial vagina and evaluated using optical microscopy for sperm motility and morphology. After evaluation, equal parts of the samples were pooled and diluted (1:9), with physiological solution-based diluters containing 0, 10%, 20% and 30% of egg white inclusion. Aliquots of 0.25 ml of diluted semen were refrigerated at 5 °C, using BotuFLEX®, so that the pool was evaluated at 0, 2, 6, 12 and 24 hours, throughout the refrigeration process. Thus, the progressive individual sperm motility of extenders with 0 and 30% inclusion showed differences (0h: 4.5 vs. 4.0; p<0.05). The egg white inclusion of 10% between the hours of 2 and 12 hours presented values above 3. As for total motility, the 10% concentration stood out statistically compared to 30% (0h: 72.5% vs. 62.5%; 24h: 30% vs. 12.5%). With regard to morphological parameters, the quantity of normal spermatozoa were statistically different for all concentrations at 0 and 24 hours. It was observed that in the flagellum, concentrations of 0 and 10% at 12 and 24 hours were statistically different at 0 hours, due to an increase of more than 20% in defects, with the exception of the 20% concentration. The ease and low cost of using saline solution stands out, estimating much more elaborate and higher-cost diluters. Thus, the use of physiological solution, whether or not added with egg white (10%) as a goat semen extender, kept under refrigeration, could be an alternative to be a low-cost and effective option