Padronização de uma tabela de escores morfológicos para avaliação do tecido testicular bovino criopreservado: uma proposta

Abstract

The creation of germplasm banks stands out primarily in the cryopreservation of gametes from endangered animals and individuals undergoing treatments such as chemotherapy and radiotherapy. Currently, cryopreservation is carried out through two main methods: slow freezing and vitrification. Despite the effectiveness of cryopreservation in addressing challenges such as infertility, it is important to note that the process can cause damage to gonadal tissue. In light of this, histological evaluation is crucial to determine the quality of tissues after cryopreservation, especially for the success of the technique. However, the morphological complexity and lack of standardization in histological assessments of testicular tissues pose persistent challenges, especially in clinical contexts. Therefore, this study proposes the development of a new scoring table for histopathological evaluation of cryopreserved bovine seminiferous tubules. The research established a standardization of histological evaluation specifically for cryopreserved bovine tissue damage, beneficial for both experienced and inexperienced evaluators. The adoption of standardized analyses contributes to increased objectivity and reduced variability in morphological results. This systematic approach promotes a solid foundation for data interpretation, enhancing reliability and applicability across investigations in this field.