Avaliação de dois métodos diagnósticos de helicobacter pylori e a correlação entre a densidade de colonização bacteriana e achados histopatológicos

Abstract

This study approaches the incidence of Helicobacter pylori infection in children and adult patients, the diagnostic performance of culture and histology, and the correlation between the density of H.pylori and histologic features. We obtained gastric biopsies from twenty-three adults an twenty- one children and submited then histopathologic and culture studies. The updated Sydney Classification System of gastritis was employed using a score of 0-3 to grade the degree and activity of inflammation and Hpylori colonization. Athophy, intestinal metaplasia and lymphoid follicles/agregates was referred as present or absent. The biopsies were evaluated by H-E and Giemsa staining. Differences in results for the culture and histologic examination were found in 13 patients and Hpylori was detected more frequently by histology. Five children (23,9%) and six adults (26,1%) were diagnosed by culture and eight children (38,1%) and sixteen adults (69,6%) by histology. All the patients who had Hpylori also presented chronic gastritis. The density of Hpylori was correlated with polymorphonuclear neutrophil activity, chronic inflammation, lymphoid follicles/agregates and positivity in culture. We concluded that Hpylori detection in routine examination is more available in histopathologic studies and that the density of Hpylori was correlated with the grade of inflammation, activity, presence of follicle lymphoid and successful culture.