Pós-condicionamento com curcumina e eletroacupuntura: efeitos sistêmicos e locais sobre a inflamação e o estresse oxidativo induzido por gentamicina no fígado em ratos wistar

Abstract

The liver acts to control and prevent the toxic effects of antibiotics administered to treat infections. Gentamicin (GM) can promote increased concentrations of reactive oxygen species, resulting in oxidative stress. Turmeric has antioxidant and antiinflammatory activity. The application of EAc to certain points in the body can reduce oxidative stress and inflammation in the liver. To evaluate the antioxidant and antiinflammatory activity of turmeric (CCM) and EAc, 56 male rats, with a mean initial weight of 126.8 g, were randomly assigned to seven groups of eight animals. G1- Saline Control, G2- Diluent Control, G3-CCM 100mg, G4-CCM 200mg, G5-EAc100 (Electroacupuncture 100 Hz), G6 – CCM 100mg+EAc 100Hz and G7-CCM 200mg+EAC. For the induction of hepatotoxicity, all animals received GM (100mg/Kg ip.) for 5 consecutive days. From the 7th day, the rats were treated with CCM and EAc. CCM was administered by gavage, every other day for 12 days. EAc was administered every other day for 12 days. The sessions lasted 20 minutes. Rats from G1 and G2 received saline (1.0ml) or diluent. Arterial blood and liver samples were collected 48 hours after the end of the experiment, under anesthesia with ketamine+xylazine via i,p. Parameters analyzed included a) Indicators of liver damage: aspartate amino transferase (AST), alanine amino transferase (ALT), gammaglutamyl transferase (GGT), blood glucose; b) indicators of oxidative stress and lipid peroxidation: substances reactive to thiobarbituric acid (TBARS), reduced glutathione (GSH) and nitrites (NO2); c) indicator of inflammation: myeloperoxidase (MPO). Oxidative liver injury was confirmed by increased AST:ALT ratio and nitrite concentrations. There was a significant increase in GSH levels, reduction in TBARS levels and MPO activity. It is concluded that GM administered at high doses (100mg/Kg) promotes oxidative and inflammatory changes in the liver of healthy rats and that turmeric and EAc exert a protective effect on oxidative stress and inflammation in the liver and plasma of rats treated with high doses of GM.